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Showing 5 results for Ghazalian

Saqqa Farajtabar Behrestaq, Nader Shakeri, Farshad Ghazalian, Hojatollah Nikbakht,
Volume 10, Issue 2 (volume 10, number 2 2018)
Abstract

Objective: MafA is one of the major factors in the family of MafA transcription factors. In pancreatic beta cells, MafA plays an important role in regulating the expression of glucose-dependent Insulin gene. On the other hand, Lipotocyticleads to negative expression of MafA expression when exposed to inflammatory cytokines. These statements in general emphasize the role that MafA plays as a key regulator of genes that are effective in maintaining the function of beta cells and glucose-dependent insulin synthesis.
Materials and Methods: Considering the very important role of MafA transcription factor in the protection and function of beta cells and insulin secretion, In order to investigate the effect of exercise activities on expression of this gene, 16 male Wistar rats were divided into diabetic control and diabetic training groups. The two groups were diabetic with receiving nicotinamide and streptozotocin injection and the experimental group was trained for 12 weeks by aerobic exercise on a treadmill.
Results: The results of the study showed a significant increase in the expression of the MafA gene after 12 weeks of aerobic training, which resulted in a significant decrease in blood glucose concentration and increased beta cell function.
Conclusion: Based on the evidence of the mechanisms responsible for the synthesis and secretion of insulin in the pancreatic beta cells, it can be concluded that increased levels of insulin levels in aerobic intervention group rats in the present study it is rooted in increasing the expression of MafA compared to the control group.
Saqqa Farajtabar Behrestaq, Nader Shakeri, Farshad Ghazalian, Hojatollah Nikbakht,
Volume 10, Issue 4 (volume 10, number 4 2018)
Abstract

Objective: Exercise as a non-pharmacological treatment plays an important role in regulating and reducing the inflammatory cytokine associated with beta cell function. Genetics is one of the most important and effective factors in the incidence of diabetes, in most cases. The present study aims to explain the effect of 12 weeks aerobic training on FOXO1 expression in pancreatic tissue, insulin and blood glucose levels in male wistar rats with type 2 diabetes mellitus.
Materials and Methods: In this study, 16 male wistar rats were divided into diabetic control and diabetic training groups. The two groups were diabetic with nicotine amide and streptozotocin injections and the training group did aerobic exercises for treadmill for 12 weeks.  
Results: The results of the study showed a significant increase in FOXO1 expression after 12 weeks of aerobic training (P-value: 0.027), which resulted in a significant decrease in blood glucose concentration (P-value: 0.0001).
Conclusion: The induction of type 2 diabetes leads to a reduction in the expression of FOXO1 gene in the tissues of the pancreas in experimental rats, which is associated with a decrease in serum levels of insulin and an increase in blood glucose levels. On the other hand, 12 weeks of aerobic training of 5 sessions per week leads to a significant increase in the expression of FOXO1 gene, with decreasing glucose and increased serum insulin in the pancreatic tissue of diabetic rats.

 
Meisam Soheil Pour, Nader Shakeri, Khosrow Ebrahim, Farshad Ghazalian,
Volume 11, Issue 4 (volume 11, number4 2019)
Abstract

Objective: The use of non-enzymatic antioxidants in the form of dietary supplements has a positive effect on reducing oxidative stress, and preventing apoptosis as they interrupt free radicals. The aim of this study was to investigate the aerobic exercise and L-carnitine consumption impacts on diabetes induced apoptosis, oxidative stress factors, and mitochondrial enzymes in rats.
Materials and Methods: In this Clinical trial study, 45 male Wistar rats (200-300 g) were divided into six groups including sham, healthy control, diabetic control, diabetic & L-carnitine, diabetic & aerobic exercise, diabetic receiving L-carnitine & aerobic exercise . The rats attained a diabetic state with a single dose of STZ intraperitoneal injection (55 mg/kg body weight) and receiving L-carnitine 100 mg per day. The aerobic exercise protocol including five sessions per week was administered. The heart tissues of the dependent variables were measured by ELISA 24 hours after the last session of the exercise program. One-way ANOVA and Tukey's post hoc test at P-value< 0.05 were used to analyze the data.
Results: result show that aerobic exercise and L-carnitine consumption have a significant effect on BAX (P-value= 0.001), Bcl-2 (P-value= 0.001), and SOD (P-value= 0.001) in diabetic rats.
Conclusion: The results of the present study confirm the role of aerobic exercise and L-carnitine in improving the indices of apoptosis and oxidative stress in type 2 diabetic rats
Seyed Esmaeil Jabbari, Mandana Gholami, Hojatollah Nikbakht, Nader Shakeri, Farshad Ghazalian,
Volume 12, Issue 1 (volume 12, number 1 2020)
Abstract

Objective: The use of non-enzymatic antioxidants in food supplements and proper exercise can have a positive effect on decreasing oxidative stress by free radical hunting. The purpose of this study was to investigate the effect of aerobic training and L-carnitine supplementation on some of the oxidative stress factors in the liver of diabetic rats.
Materials and Methods: In this experimental study, 45 male wistar rats (200-300 gr) were randomly divided into six groups: 1) sham group, 2) healthy control group 3) diabetic control group, 4) diabetic group receiving L-carnitine, 5) diabetic group of aerobic training, 6) diabetic group of aerobic training and receiving L-carnitine. The aerobic exercise protocol included six weeks, five sessions per week on the treadmill. After intervention, malondialdehyde (MDA), superoxide dismutase (SOD), and glutathione peroxidase (GPX) levels were determined in liver tissue.
Results: Six weeks of aerobic exercise had a significant effect on MDA factor in hepatic tissue in diabetic rats (P-value: 0.024). However, supplementation (P-value: 0.868) and combined intervention of aerobic exercise and supplementation of L-carnitine (P-value: 0.465) did not have the significant effect on MDA factor. Also, 6 weeks of aerobic training, supplementation of L-carnitine, and combined intervention had no significant effect on SOD and GPX factors of hepatic tissue in diabetic rats (P-value> 0.05).
Conclusion: L-carnitine supplementation with regular exercise can have beneficial effects on hepatic antioxidant defense system in rats with type 2 diabetes.
Fariba Bakhtiari, Hasan Matin Homaee, Farshad Ghazalian,
Volume 12, Issue 2 (volume 12, number 2 2020)
Abstract

Objective: The goal of this research was to determine the influence of 4 weeks aerobic training on gene expression of tumor necrosis factor alpha (TNF-α), hypoxia-inducible factor-1 alpha (HIF-1α) and vascular endothelial growth factor (VEGF) in the cardiac tissue of diabetic rats.
Materials and Methods: In an experimental study, 30 male wistar rats were partitioned into three groups (n=10), diabetic aerobic training, diabetic control and healthy control (n=10). Induction of type 2diabetes (T2DM) was done by intraperitoneal infusion of streptozotocin. The progressive aerobic training protocol entailed 4 weeks, 5 sessions per week running on treadmill at velocity of 20 m/min for 60 min. The gene expression of TNF-α, VEGF and HIF-1α were measured by real time & PCR. One way analysis of variance and bonferroni test were applied to analysis the data. The significant level was set at P-value< 0.05.
Results: The results indicated that the aerobic training induced significant decrease in TNF-α mRNA (P-value< 0.001) and significant increase in HIF-1α mRNA (P-value< 0.001) and VEGF mRNA (P-value< 0.001) compared to diabetic control group.
Conclusion: It appears that aerobic training with reduction of TNF-α issues an affirmative effect on angiogenesis, as a result, it improves diabetic cardiac.

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