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Showing 5 results for Mohiti-Ardekani

Javad Mohiti-Ardekani, Fria Talebi, Mohammad Afkhami-Ardekani,
Volume 1, Issue 1 (9-2009)

INTRODUCTION: Leptin is a hormone resulting from the obesity gene which could be important in the pathogenesis of the insulin. Only limited data concerning the interaction of insulin, glucose tolerance and free leptin are available and no data exist on the potential influence of free vs. bound circulating leptin. We, therefore, studied percentage of free to total leptin in diabetic patients. MATERIAL AND METHODS: Thirty non-insulin dependent diabetic obese patients (age: 50±20 years, BMI >30 kg/m²) and 30 non-insulin dependent diabetic non-obese patients (age: 50±20 years, BMI <25 kg/m²) were studied. Free leptin was purified by Gel filtration chroma-tography and the fractions were collected and then their free leptin was measured by a high sensitive ELISA method. Circulation total leptin and insulin was measured by ELISA. RESULTS: Circulation total leptin was significantly correlated to insulin (P < 0.005). Percen-tage of free leptin to total in obese subjects was more than non-obese subjects (27% ± %1 vs. %3 ± %4, [P < 0.001]). Percentage of free to total leptin showed a positive correlation with insulin (r = 0.58 [P < 0.001]), insulin resistance (r = 31 [P < 0.015]) and BMI (r = 0.86 [P < 0.001]). CONCLUSION: The majority of leptin which circulates in obese individuals was free form. Presumably it is bioactive protein of hormone and thus obese subjects are resistant to free leptin.
Javad Mohiti-Ardekani, Zahra Akbarian, Mohammad.reza Piri-Ardekani, Azra Mohiti-Ardekani,
Volume 4, Issue 2 (6-2012)

Objectives: Diabetes is one of the most common metabolic diseases in the world. It affects 6.6% of world population and about 3 million individuals in Iran. Nowadays chemical and herbal medicines are widely prescribed to cure obesity. In Iran, cumin (Cuminum Cyminum L.) is a plant used in traditional medicine to cure obesity, and some of the new studies have suggested that cumin has a role in diabetes treatment and also in reducing the lipids level. In this study, we investigated the cumin oil and sibutramine effect on the prevention of weight gain and the level of serum leptin, glucose, and lipids in normal Wistar rats. Materials and methods: We divided 36 male rats of Wistar race into 3 equal groups: the control group with normal regimen, the cumin oil group with normal regimen, the sibutramine group with normal regimen. The consumed dosages of cumin oil and sibutramine were 400 µg/kg and 3mg/kg respectively which was given to the rats by gavage (tube feeding). In this study, we took samples of the hungry rats during three various periods including the first day of the study, 20th day (the beginning of the medicine usage) and 55th day (the end of the medicine usage) in order to measure their glucose and serum lipids. Results: The results of this study showed a significant decrease in glucose, cholesterol, triglyceride, LDL (P<0.001) and a significant increase in serum HDL (P=0.05). Both drugs prevented weight gain at the end of study (P=0.05). Conclusion: The findings indicate that cumin oil like sibutramine via consumption by gavage can affect the serum glucose and lipids in rats and also prevent weight gain.
Ali Moradi, Mohammad Bahrami, Gilda Eslami, Javad Mohiti-Ardekani,
Volume 6, Issue 2 (volume 6, number2 2014)

Objective: Adipocyte and skeletal muscle are important tissues which contribute the development and progression of metabolic disorder. Insulin has a major regulatory function on glucose metabolism in these tissues by redistributing glucose transporter (GLUT4) from intracellular vesicles to the cell surface. Today, due to the side effects of chemical medications attendance to herbal medicines is growing. In this study, the effect of Curcumin extract as main polyphenols in Turmeric on gene expression of GLUT4 was evaluated. Materials and Methods: Curcumin was extracted using alcohol and chloroform from turmeric powder.TLC chromatography was used to confirm purity of Curcumin extracted. Mouse C2C12 myoblasts were grown in Dulbecco’s Modified Eagle Medium (DMEM 1.5 g/l glucose) supplemented with 10% fetal bovine serum, 50 U/ml penicillin, and 50 μg/ml streptomycin. After differentiating of C2C12 cells during 4 days to myotubes, cells were treated separately in the presence of insulin (100 nM), Curcumin (25 µM) and co-treatment for 24h.RNA extraction from C2C12 cells was performed and GLUT4 expression levels were examined by semi-quantitative RT-PCR. Results: The results showed a significant increase in the GLUT4expression in Curcumin treatment group, in compare with negative control but less than insulin as a positive control. The synergistic effect of Curcumin and insulin was lower in comparison with insulin as a positive control. Conclusion: Curcumin could increase the gene expression but synergistic effects of Curcumin and insulin is more powerful than Insulin which can be due to the competitive action of insulin and Curcumin in activation of gene expression pathway.
Zahra Malekpour-Dehkordi, Javad Mohiti-Ardekani, Yousof Naghiaee, Shahram Teimourian, Mahdie Hemati, Mitra Nourbakhsh,
Volume 12, Issue 4 (volume 12, number 4 2020)

 Objective: In obesity, chronic low grade inflammation, created by induction of pro-inflammatory markers, causes adipocyte dysfunction in adipose tissue. Adipocytes dysfunction is associated with various diseases including insulin resistance and obesity. In obesity, inflammatory factors such as osteopontin (OPN), angiopoietin-like protein 2 (Angptl2) and transforming growth factor-β (TGF-β) are induced in adipose tissue. Metformin and pioglitazone that are used to modulate inflammation, but the relevant mechanism is poorly understood. This study aimed to investigate the effect of metformin and pioglitazone as anti-diabetic drugs, on gene expression of osteopontin, Angptl2 and TGF-β as inflammatory factors in insulin resistance and hypertrophied adipocyte in 3T3-L1 cell line model in vitro.
Materials and Methods: In this experimental research, we differentiated3T3-L1 preadipocytes to adipocytes. The adipocytes treated in insulin resistance and hypertrophied conditions with metformin and pioglitazone and assayed gene expression of OPN, Angptl2 and TGF-β by Real-Time PCR. Data was analyzed by SPSS statistic software.
Results: The results showed that expression of OPN, Angptl2, and TGF-β were increased significantly over 2-fold (P-value< 0.05) in insulin resistance and hypertrophied adipocytes compared to normal adipocytes. Pre- and co-treatment with metformin and pioglitazone led to reduced expression of Angptl2 and TGF-β. Only metformin significantly reduced the expression of Angptl2, TGF-β and OPN in hypertrophied adipocyte.
Conclusion: These results support the proposal that metformin and pioglitazone reduce gene expression of inflammatory factors in insulin resistant and hypertrophied adipocytes.
Reza Didehdar, Yousof Naghiaee, Javad Mohiti-Ardekani, Naeimeh Heiranizadeh, Masaoud Rahmanian,
Volume 13, Issue 2 (volume 13, number 2 2021)

Objective: Obesity is a factor in the development of insulin resistance and type 2 diabetes. Obesity contributes a wide variety of metabolic changes such as insulin resistance. The insulin signal mechanism to intra-cells occurs in insulin resistance, primarily in adipose tissue cells, which can be appropriate targets for therapeutic approaches by recognizing the proteins in this pathway. The study aimed to evaluate the simultaneous impact of metformin and sitagliptin on the expression of protein levels involved in insulin resistance Protein Kinase B (Akt) and Glucose Transporter 4 (GLUT4) in diabetic adipose tissue.
Materials and Methods: In order to evaluate the content of proteins involved in insulin resistance Akt and GLUT4 in adipose tissue of diabetic patients with the use of SDS-PAGE and western blot analyses, we studied 6 persons of type 2 diabetic patients who obtained 3 months of care with simultaneous metformin and sitagliptin, 4 persons returned from them after treatment and 8 persons as a stable case (control group).
Results: There was an increase in glucose intake and a decrease in serum glucose levels (P-value= 0.025) and no decrease in insulin resistance (P-value= 0.6) following simultaneous metformin and sitagliptin therapy, but no improvement in serum insulin levels (P-value=1.01). Increases in the content of Akt protein (P-value= 0.682) and GLUT4 protein (P-value= 0.851) involved in insulin resistance in diabetic patients' adipose tissue, were not observed.
Conclusion: Simultaneous treatment with metformin and sitagliptin had no effect on insulin resistance proteins Akt and GLUT4 in type 2 diabetic adipose tissue.

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