Iranian Journal of Diabetes and Obesity
Shahid Sadoughi University Of Medical Sciences
Sun, Dec 22, 2024
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Volume 13, Issue 3 (9-2021)
IJDO 2021, 13(3): 160-165 |
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Nadi M, Banaeifar A, Arshadi S. Effect of an Aerobic Exercise Course on PI3K and AKT1 Expression and Neural Muscle Insulin Resistance in Diabetic Rats. IJDO 2021; 13 (3) :160-165
URL: http://ijdo.ssu.ac.ir/article-1-647-en.html
URL: http://ijdo.ssu.ac.ir/article-1-647-en.html
PHD Student, Islamic Azad University of Tehran South, Tehran, Iran.
Abstract: (1120 Views)
Objective: The purpose of the present study was to investigate eight weeks of aerobic workout on PI3K and AKT expression as well as insulin resistance (IR) of muscle in diabetic rats by nicotinamide - streptozotocin.
Materials and Methods: This laboratory study was conducted on 14 male Wistar rats (8 to 10 weeks) with a weight range of 201 to 250 g and induction of type 2 diabetes (one week). These mice were classified into 2 groups: aerobic training and control group. No exercise was given to the control group during the study, while the aerobic exercise program was run for 5 weeks a week with a gradual increase of speed (10 to 25 m / min) and time (15 to 40 minutes) in running treadmill was performed for the aerobic training group. Assay of gene expression in both study groups was by rt-PCR. For statistical analysis, the SPSS 19 software was used. The variables were compared between the two groups using one-way ANOVA.
Results: The findings showed that there was no significant difference between the two groups in terms of IR in diabetic rats (3.85 (± 0.39) vs. 5.26 (± 0.55); P-value= 0.345). The expression of AKT (2.37 (± 2.33) vs. 1.000 (± 0.001); P-value= 0.042) and PI3K (2.87 (± 2.54) vs. 1.000 (± 0.001); P-value= 0.028) in the neural muscle of the training group compared with control group had a significant increase.
Conclusion: It seems that performing eight weeks of aerobic exercise could be a strong stimulus for PI3K and AKT gene expression.
Materials and Methods: This laboratory study was conducted on 14 male Wistar rats (8 to 10 weeks) with a weight range of 201 to 250 g and induction of type 2 diabetes (one week). These mice were classified into 2 groups: aerobic training and control group. No exercise was given to the control group during the study, while the aerobic exercise program was run for 5 weeks a week with a gradual increase of speed (10 to 25 m / min) and time (15 to 40 minutes) in running treadmill was performed for the aerobic training group. Assay of gene expression in both study groups was by rt-PCR. For statistical analysis, the SPSS 19 software was used. The variables were compared between the two groups using one-way ANOVA.
Results: The findings showed that there was no significant difference between the two groups in terms of IR in diabetic rats (3.85 (± 0.39) vs. 5.26 (± 0.55); P-value= 0.345). The expression of AKT (2.37 (± 2.33) vs. 1.000 (± 0.001); P-value= 0.042) and PI3K (2.87 (± 2.54) vs. 1.000 (± 0.001); P-value= 0.028) in the neural muscle of the training group compared with control group had a significant increase.
Conclusion: It seems that performing eight weeks of aerobic exercise could be a strong stimulus for PI3K and AKT gene expression.
Type of Study: Research |
Subject:
Special
Received: 2021/09/14 | Accepted: 2021/09/19 | Published: 2021/09/19
Received: 2021/09/14 | Accepted: 2021/09/19 | Published: 2021/09/19
References
1. Rask-Madsen C, King GL. Vascular complications of diabetes: mechanisms of injury and protective factors. Cell metabolism. 2013;17(1):20-33. [DOI:10.1016/j.cmet.2012.11.012]
2. Huang X, Liu G, Guo J, Su Z. The PI3K/AKT pathway in obesity and type 2 diabetes. International journal of biological sciences. 2018;14(11):1483. [DOI:10.7150/ijbs.27173]
3. Shiojima I, Walsh K. Regulation of cardiac growth and coronary angiogenesis by the Akt/PKB signaling pathway. Genes & development. 2006;20(24):3347-65. [DOI:10.1101/gad.1492806]
4. Cai S, Khoo J, Channon KM. Augmented BH4 by gene transfer restores nitric oxide synthase function in hyperglycemic human endothelial cells. Cardiovascular research. 2005;65(4):823-31. [DOI:10.1016/j.cardiores.2004.10.040]
5. Yang Z, Scott CA, Mao C, Tang J, Farmer AJ. Resistance exercise versus aerobic exercise for type 2 diabetes: a systematic review and meta-analysis. Sports medicine. 2014;44(4):487-99. [DOI:10.1007/s40279-013-0128-8]
6. Azari N, Rahmati M, Fathi M. The Effects Of Endurance Exercise On Blood Glucose, Insulin And Insulin Resistance In Patients With Type II Diabetes: A Systematic Review And Meta-Analysis Of Studies In Iran. Iranian Journal Of Diabetes And Metabolism. 2018;17(2):65-78.
7. Ivy JL. Role of exercise training in the prevention and treatment of insulin resistance and non-insulin-dependent diabetes mellitus. Sports medicine. 1997;24(5):321-36. [DOI:10.2165/00007256-199724050-00004]
8. Suhara T, Baba Y, Shimada BK, Higa JK, Matsui T. The mTOR signaling pathway in myocardial dysfunction in type 2 diabetes mellitus. Current diabetes reports. 2017;17(6):38. [DOI:10.1007/s11892-017-0865-4]
9. Marliss EB, Vranic M. Intense exercise has unique effects on both insulin release and its roles in glucoregulation: implications for diabetes. Diabetes. 2002;51(1):S271-83. [DOI:10.2337/diabetes.51.2007.S271]
10. Crane JD, MacNeil LG, Tarnopolsky MA. Long-term aerobic exercise is associated with greater muscle strength throughout the life span. Journals of Gerontology Series A: Biomedical Sciences and Medical Sciences. 2013;68(6):631-8. [DOI:10.1093/gerona/gls237]
11. Li J, Liu Y, Liu B, Li F, Hu J, Wang Q, et al. Mechanisms of aerobic exercise upregulating the expression of hippocampal synaptic plasticity-associated proteins in diabetic rats. Neural plasticity. 2019;2019. [DOI:10.1155/2019/7920540]
12. Kazior Z, Willis SJ, Moberg M, Apro W, Calbet JA, Holmberg HC, et al. Endurance exercise enhances the effect of strength training on muscle fiber size and protein expression of Akt and mTOR. PloS one. 2016;11(2):e0149082. [DOI:10.1371/journal.pone.0149082]
13. Chavanelle V, Boisseau N, Otero YF, Combaret L, Dardevet D, Montaurier C, et al. Effects of high-intensity interval training and moderate-intensity continuous training on glycaemic control and skeletal muscle mitochondrial function in db/db mice. Scientific reports. 2017;7(1):1-10. [DOI:10.1038/s41598-017-00276-8]
14. Lin CH, Lin CC, Ting WJ, Pai PY, Kuo CH, Ho TJ, et al. Resveratrol enhanced FOXO3 phosphorylation via synergetic activation of SIRT1 and PI3K/Akt signaling to improve the effects of exercise in elderly rat hearts. Age. 2014;36(5):1-0. [DOI:10.1007/s11357-014-9705-5]
15. SherafatiMoghadam M, Salesi M, Daryanoosh F, HematiNafar M, Fallahi A. The effect of 4 weeks of high intensity interval training on the content of AKT1, mTOR, P70S6K1 and 4E-BP1 in soleus skeletal muscle of rats with type 2 diabetes: An experimental study. Journal of Rafsanjan University of Medical Sciences. 2018;17(9):843-54. (in Persian)
16. Mascher H, Ekblom B, Rooyackers O, Blomstrand E. Enhanced rates of muscle protein synthesis and elevated mTOR signalling following endurance exercise in human subjects. Acta Physiologica. 2011;202(2):175-84. [DOI:10.1111/j.1748-1716.2011.02274.x]
17. Shadmehri S, Moghadam MS, Daryanoosh F, Golbar SJ, Tanideh N. The effect of 8 weeks endurance exercise on the content of total and phosphorylated AKT1, mTOR, P70S6K1 and 4E-BP1 in skeletal muscle FHL of rats with type 2 diabetes. Journal of Shahid Sadoughi University of Medical Sciences. 2019;26(12):1063-74. (in Persian) [DOI:10.18502/ssu.v26i12.663]
18. Zhang BB, Zhou G, Li C. AMPK: an emerging drug target for diabetes and the metabolic syndrome. Cell metabolism. 2009;9(5):407-16. [DOI:10.1016/j.cmet.2009.03.012]
19. Christ CY, Hunt D, Hancock J, Garcia-Macedo R, Mandarino LJ, Ivy JL. Exercise training improves muscle insulin resistance but not insulin receptor signaling in obese Zucker rats. Journal of Applied Physiology. 2002;92(2):736-44. [DOI:10.1152/japplphysiol.00784.2001]
20. Banks EA, Brozinick Jr JT, Yaspelkis III BB, Kang HY, Ivy JL. Muscle glucose transport, GLUT-4 content, and degree of exercise training in obese Zucker rats. American Journal of Physiology-Endocrinology And Metabolism. 1992;263(5):E1015-20. [DOI:10.1152/ajpendo.1992.263.5.E1015]
21. Brozinick Jr JT, Etgen Jr GJ, Yaspelkis 3rd BB, Kang HY, Ivy JL. Effects of exercise training on muscle GLUT-4 protein content and translocation in obese Zucker rats. American Journal of Physiology-Endocrinology And Metabolism. 1993;265(3):E419-27. [DOI:10.1152/ajpendo.1993.265.3.E419]
22. Radak Z, Kumagai S, Nakamoto H, Goto S. 8-Oxoguanosine and uracil repair of nuclear and mitochondrial DNA in red and white skeletal muscle of exercise-trained old rats. Journal of Applied Physiology. 2007 Apr;102(4):1696-701.. [DOI:10.1152/japplphysiol.01051.2006]
23. Gougeon R, Morais JA, Chevalier S, Pereira S, Lamarche M, Marliss EB. Determinants of whole-body protein metabolism in subjects with and without type 2 diabetes. Diabetes care. 2008;31(1):128-33. [DOI:10.2337/dc07-1268]
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