The Effect of Endurance Training on Expression of Autophagy Genes (Beclin-1, ULK-1) in the Heart Tissue of Male Rats with Experimental Diabetes

Hosseinzadeh, Masoumeh; Taheri, Asma

doi:10.18502/ijdo.v14i1.8742

Volume 14, Issue 1 (volume 14, number 1 2022) IJDO 2022, 14(1): 51-54 | Back to browse issues page

‎ 10.18502/ijdo.v14i1.8742

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Hosseinzadeh M, Taheri A. The Effect of Endurance Training on Expression of Autophagy Genes (Beclin-1, ULK-1) in the Heart Tissue of Male Rats with Experimental Diabetes. IJDO 2022; 14 (1) :51-54
URL: http://ijdo.ssu.ac.ir/article-1-687-en.html

The Effect of Endurance Training on Expression of Autophagy Genes (Beclin-1, ULK-1) in the Heart Tissue of Male Rats with Experimental Diabetes

Masoumeh Hosseinzadeh ^*

, Asma Taheri

Instructor of Department of Sport Physiology, Faculty of Sport Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran.

Abstract: (973 Views)

Objective: This study aimed to investigate the effect of six weeks of endurance training on the expression of autophagy-related genes (Beclin-1, ULK-1) in the heart tissue (Myocardium) of rats with diabetes.
Materials and Methods: Twenty male wistar rats (weight: 204±11.3g), (age= 8 weeks) were divided into four groups including: 1) diabetic type1 training, group 2) diabetic type1 control, group 3) healthy training group, and 4) healthy control group. They developed diabetes by intraperitoneal injection of streptozotocin (STZ) (45 mg / kg). The endurance training was performed with a moderate intensity (50-55% vo2max) of 5 days a week for 6 weeks. The expression level of autophagy-related genes (ULK-1, Beclin-1) was measured by real-time technique. One-way analysis of variance and Tukey's post hoc test were used for statistical analysis.
Results: The expression of autophagy genes (Beclin-1: diabetic type1 control group: 2.49 (±0.53), and healthy control group: 1 (±0.21)), (ULK-1; diabetic type1 control group: 2.56 (±1.02), and healthy control group: 1 (±0.35)) in heart tissue in diabetic rats was significantly higher than in the healthy control group (P: 0.001). After six weeks of endurance training, the expression of these genes in heart tissue was significantly lower in the diabetic training group, healthy training and healthy control than in the diabetic control group. (P: 0.001).
Conclusion: The present study results showed that endurance training by reducing autophagy factors in the heart of male rats with diabetes could be helpful as a preventive and non-pharmacological agent in the treatment of diabetic patients.

Keywords: Endurance training, Beclin-1, ULK-1, Diabetes, Heart tissue

Full-Text [PDF 386 kb] (359 Downloads)

Type of Study: Research | Subject: Special
Received: 2021/10/12 | Accepted: 2022/01/11 | Published: 2022/02/26

References

1. Mizushima N, Komatsu M. Autophagy: renovation of cells and tissues. Cell. 2011;147(4):728-41. [DOI:10.1016/j.cell.2011.10.026]

2. Zhao L, Ackerman SL. Endoplasmic reticulum stress in health and disease. Current opinion in cell biology. 2006;18(4):444-52. [DOI:10.1016/j.ceb.2006.06.005]

3. Yan JE, Yuan W, Lou X, Zhu T. Streptozotocin-induced diabetic hyperalgesia in rats is associated with upregulation of Toll-like receptor 4 expression. Neuroscience letters. 2012;526(1):54-8. [DOI:10.1016/j.neulet.2012.08.012]

4. Morrow TJ. Animal models of painful diabetic neuropathy: the STZ rat model. Current protocols in neuroscience. 2004;29(1):9-18. [DOI:10.1002/0471142301.ns0918s29]

5. Wei M, Ong L, Smith MT, Ross FB, Schmid K, Hoey AJ, et al. The streptozotocin-diabetic rat as a model of the chronic complications of human diabetes. Heart, Lung and Circulation. 2003;12(1):44-50. [DOI:10.1046/j.1444-2892.2003.00160.x]

6. Chae CH, Jung SL, An SH, Jung CK, Nam SN, Kim HT. Treadmill exercise suppresses muscle cell apoptosis by increasing nerve growth factor levels and stimulating p-phosphatidylinositol 3-kinase activation in the soleus of diabetic rats. Journal of physiology and biochemistry. 2011;67(2):235-41. [DOI:10.1007/s13105-010-0068-9]

7. Jokar M, Sherafati-Moghadam M. The effect of 4 weeks high intensity interval training (HIIT) on the content of FOXO3a Beclin-1 proteins in the left ventricular heart tissue with type 2 diabetic rats. Journal of Kashan University of Medical Sciences (FEYZ). 2020;24(2):160-9. https://feyz.kaums.ac.ir/ article-1-3975-en.html. (in Persian)

8. Munasinghe PE, Riu F, Dixit P, Edamatsu M, Saxena P, Hamer NS, et al. Type-2 diabetes increases autophagy in the human heart through promotion of Beclin-1 mediated pathway. International journal of cardiology. 2016;202:13-20. [DOI:10.1016/j.ijcard.2015.08.111]

9. Gao H, Yang Q, Dong R, Hou F, Wu Y. Sequential changes in autophagy in diabetic cardiac fibrosis. Molecular medicine reports. 2016;13(1):327-32. [DOI:10.3892/mmr.2015.4517]

10. Jung HS, Chung KW, Kim JW, Kim J, Komatsu M, Tanaka K, et al. Loss of autophagy diminishes pancreatic β cell mass and function with resultant hyperglycemia. Cell metabolism. 2008;8(4):318-24. [DOI:10.1016/j.cmet.2008.08.013]

11. Zarghami KA, Nikookheslat S, Karimi P. The Effect Of High-Intensity Interval Training (Hiit) With And Without Caffeine Injection On Expression Of Myocardial Autophagy-Related Proteins In Diabetic Rats. Journal of Diabetes and Metabolic Disorders. 2020;19(4):183-94. (in Persian)