Iranian Journal of Diabetes and Obesity
Shahid Sadoughi University Of Medical Sciences
Mon, Dec 8, 2025
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Volume 17, Issue 4 (10-2025)
IJDO 2025, 17(4): 256-265 |
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Tigstu Ayele F, Kucha Dewelle W, Degef Teklemariam M, Natesan G, Bekuretsion Abraham Y, Woredebrhan Mesfin G, et al . Anti-Hyperglycemic Role of Hydroethanolic Extract of Foeniculum Vulgare Seed on Diabetic Wistar Albino Rats. IJDO 2025; 17 (4) :256-265
URL: http://ijdo.ssu.ac.ir/article-1-993-en.html
URL: http://ijdo.ssu.ac.ir/article-1-993-en.html
Feven Tigstu Ayele * 
, Winner Kucha Dewelle , Maria Degef Teklemariam , Gnanasekaran Natesan , Yonas Bekuretsion Abraham , Gelila Woredebrhan Mesfin , Chala Bashea Hailu , Solomon Genet Gebre
, Winner Kucha Dewelle , Maria Degef Teklemariam , Gnanasekaran Natesan , Yonas Bekuretsion Abraham , Gelila Woredebrhan Mesfin , Chala Bashea Hailu , Solomon Genet Gebre
Department of Biomedical, Dire Dawa University, Dire Dawa, Ethiopia.
Abstract: (239 Views)
Objective: Foeniculum vulgare has been suggested as a potential option for the management of hyperglycemia. This study aimed to evaluate the anti-hyperglycemic effects of the hydro-ethanolic extract of Foeniculum vulgare seeds (HEEFVS) in alloxan-induced diabetic rats.
Materials and Methods: Thirty-six male rats were randomly divided into six groups (I–VI). Diabetes was induced by intraperitoneal injection of alloxan (175 mg/kg) in groups II–VI. Groups I and II received distilled water; groups III, IV, and V were treated with HEEFVS at doses of 100, 200, and 400 mg/kg body weight/day, respectively; and group VI received glibenclamide (5 mg/kg body weight/day). Serum glucose, total cholesterol (TC), triglycerides (TG), aspartate transaminase (AST), alanine transaminase (ALT), and histological analysis of the liver were assessed.
Results: Administration of HEEFVS at 200 and 400 mg/kg significantly decreased ALT and AST activities (P< 0.001). Total cholesterol levels were significantly reduced (P< 0.001) at all tested doses (100, 200, and 400 mg/kg). Triglyceride levels showed a significant reduction (P< 0.01) only at the 400 mg/kg dose. During the second and third weeks, administration of 200 and 400 mg/kg markedly reduced glucose levels (P< 0.001). Complete regeneration of liver tissue was observed in the 400 mg/kg group, while partial restoration was seen in the 100 mg/kg and 200 mg/kg groups. DPPH radical scavenging and α-amylase inhibitory activities of HEEFVS demonstrated IC50 values of 146.6 µg/ml and 8.47 µg/ml, respectively. The LD50 of HEEFVS was greater than 3500 mg/kg.
Conclusion: HEEFVS exhibits strong hepato-protective, hypolipidemic and anti-hyperglycemic effects.
Materials and Methods: Thirty-six male rats were randomly divided into six groups (I–VI). Diabetes was induced by intraperitoneal injection of alloxan (175 mg/kg) in groups II–VI. Groups I and II received distilled water; groups III, IV, and V were treated with HEEFVS at doses of 100, 200, and 400 mg/kg body weight/day, respectively; and group VI received glibenclamide (5 mg/kg body weight/day). Serum glucose, total cholesterol (TC), triglycerides (TG), aspartate transaminase (AST), alanine transaminase (ALT), and histological analysis of the liver were assessed.
Results: Administration of HEEFVS at 200 and 400 mg/kg significantly decreased ALT and AST activities (P< 0.001). Total cholesterol levels were significantly reduced (P< 0.001) at all tested doses (100, 200, and 400 mg/kg). Triglyceride levels showed a significant reduction (P< 0.01) only at the 400 mg/kg dose. During the second and third weeks, administration of 200 and 400 mg/kg markedly reduced glucose levels (P< 0.001). Complete regeneration of liver tissue was observed in the 400 mg/kg group, while partial restoration was seen in the 100 mg/kg and 200 mg/kg groups. DPPH radical scavenging and α-amylase inhibitory activities of HEEFVS demonstrated IC50 values of 146.6 µg/ml and 8.47 µg/ml, respectively. The LD50 of HEEFVS was greater than 3500 mg/kg.
Conclusion: HEEFVS exhibits strong hepato-protective, hypolipidemic and anti-hyperglycemic effects.
Keywords: Alloxan, Diabetes mellitus, Wistar albino rats, Foeniculum vulgare, Anti-hyperglycemic effect
Type of Study: Research |
Subject:
Special
Received: 2025/10/26 | Accepted: 2025/12/23 | Published: 2025/12/23
Received: 2025/10/26 | Accepted: 2025/12/23 | Published: 2025/12/23
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