Showing 2 results for Turmeric
Fereshteh Ahmadipour, Tahereh Vakili, Abdolrahim Absalan, Javad Mohiti-Ardakani, Hosein Hadinedoushan, Mohammadali Khalili, Fatemeh Pourrajab,
Volume 4, Issue 4 (10-2012)
Abstract
Objective: GLUT4 is a type of glucose transporter and plays a central role in whole-body metabolism of carbohydrates. The muscle is the major site of GLUT4 and cell line models, to explore GLUT4 behaviors under new therapeutic approach, such as herbal components, should be evaluated. Here, C2C12 cell line is evaluated for GLUT4 translocation from intracellular compartment into the cell membrane.
Materials and Methods: C2C12 cell lines were cultured and differentiated into myotubes. Cinnamon/Turmeric-water soluble extract (CWE and TWE) were prepared and differentiated myotubes were exposed to the 100, 1000 μg/ml CWE, 100 μg/ml TWE or dimethylsulfoxide for 3 hours. Intracellular/cytoplasmic membrane compartments were separated using ultracentrifugation. GLUT4 percentages quantities were measured using western blotting. Data analysis of 3 sample sizes was done by comparing mean±SD of GLUT4 quantities and independent samples t-test.
Results: CWE enhances GLUT4 translocation from intracellular compartment into the cytoplasmic membrane its effect is also dose-dependent meaning that 1000 μg/ml concentration has a more potent effect than 100 μg/ml (p<0.05). However, 100 μg/ml TWE had a reverse effect (p<0.05).
Conclusion: Here we have shown that C2C12 is a good model for exploring GLUT4 changes under the effect of herbal extract. Induction or blockade of GLUT4 maybe under control of different signals transduction pathways. Furthermore, although turmeric ingredients are declared to have somewhat anti-diabetic effects, here we have shown that such effect is not applied via TWE effects on GLUT4 intracellular compartments movement into the cell membrane.
Somaye Fakhri, Saeed Shakeryan, Aliakbar Alizadeh, Ali Shahryari,
Volume 11, Issue 3 (9-2019)
Abstract
Objective: Thepurpose of this study was to investigate the effect of 6 weeks of HIIT training combining nano-curcumin supplement on antioxidant defense and lipid degradation in overweight girls.
Materials and Methods: The research method was semi-experimental study. Accordingly, 48 overweight girl students were randomly divided into four groups: training (n=12), training-supplement (n=12), supplement (n=12) and control group (n=12). Supplement groups consumed 80 mg nano-curcumin capsule daily. Training groups performed an exercise protocol of HIIT training with maximum heart rate for 6 weeks (three sessions per week). The control group did not have any regular exercise. Blood samples were obtained before and after training period for antioxidant indicators and lipid degradation measurement. T-test and one-way analysis of variance were used for the evaluation of within-group and between-group differences, respectively.
Results: A significant increase was observed in serum levels of Malondialdehyde (P-value= 0.004) in the training group after 6 weeks. Also, there was a significant increase in serum Glutathione (P-value= 0.001), Superoxide dismutase (P-value= 0.006) and Catalase indexes (P-value= 0.01) in the supplement group. Moreover, a significant increase in catalase (P-value= 0.001), glutathione (P-value= 0.006), superoxide dismutase (P-value= 0.015) and glutathione peroxidase indexes (P-value= 0.05) and a significant decrease in malondialdehyde (P-value= 0.009) were observed in the training supplement group.
Conclusion: A positive antioxidant effect was seen, so taking curcumin supplement along with exercises may have beneficial effects on reinforcement the antioxidant system and prevention of lipid peroxidation in overweight individuals.
