Fereshteh Ahmadipour, Tahereh Vakili, Abdolrahim Absalan, Javad Mohiti-Ardakani, Hosein Hadinedoushan, Mohammadali Khalili, Fatemeh Pourrajab,
Volume 4, Issue 4 (10-2012)
Abstract
Objective: GLUT4 is a type of glucose transporter and plays a central role in whole-body metabolism of carbohydrates. The muscle is the major site of GLUT4 and cell line models, to explore GLUT4 behaviors under new therapeutic approach, such as herbal components, should be evaluated. Here, C2C12 cell line is evaluated for GLUT4 translocation from intracellular compartment into the cell membrane.
Materials and Methods: C2C12 cell lines were cultured and differentiated into myotubes. Cinnamon/Turmeric-water soluble extract (CWE and TWE) were prepared and differentiated myotubes were exposed to the 100, 1000 μg/ml CWE, 100 μg/ml TWE or dimethylsulfoxide for 3 hours. Intracellular/cytoplasmic membrane compartments were separated using ultracentrifugation. GLUT4 percentages quantities were measured using western blotting. Data analysis of 3 sample sizes was done by comparing mean±SD of GLUT4 quantities and independent samples t-test.
Results: CWE enhances GLUT4 translocation from intracellular compartment into the cytoplasmic membrane its effect is also dose-dependent meaning that 1000 μg/ml concentration has a more potent effect than 100 μg/ml (p<0.05). However, 100 μg/ml TWE had a reverse effect (p<0.05).
Conclusion: Here we have shown that C2C12 is a good model for exploring GLUT4 changes under the effect of herbal extract. Induction or blockade of GLUT4 maybe under control of different signals transduction pathways. Furthermore, although turmeric ingredients are declared to have somewhat anti-diabetic effects, here we have shown that such effect is not applied via TWE effects on GLUT4 intracellular compartments movement into the cell membrane.
Ali Moradi, Mohammad Bahrami, Gilda Eslami, Javad Mohiti-Ardekani,
Volume 6, Issue 2 (6-2014)
Abstract
Objective: Adipocyte and skeletal muscle are important tissues which contribute the development and progression of metabolic disorder. Insulin has a major regulatory function on glucose metabolism in these tissues by redistributing glucose transporter (GLUT4) from intracellular vesicles to the cell surface. Today, due to the side effects of chemical medications attendance to herbal medicines is growing. In this study, the effect of Curcumin extract as main polyphenols in Turmeric on gene expression of GLUT4 was evaluated.
Materials and Methods: Curcumin was extracted using alcohol and chloroform from turmeric powder.TLC chromatography was used to confirm purity of Curcumin extracted. Mouse C2C12 myoblasts were grown in Dulbecco’s Modified Eagle Medium (DMEM 1.5 g/l glucose) supplemented with 10% fetal bovine serum, 50 U/ml penicillin, and 50 μg/ml streptomycin. After differentiating of C2C12 cells during 4 days to myotubes, cells were treated separately in the presence of insulin (100 nM), Curcumin (25 µM) and co-treatment for 24h.RNA extraction from C2C12 cells was performed and GLUT4 expression levels were examined by semi-quantitative RT-PCR.
Results: The results showed a significant increase in the GLUT4expression in Curcumin treatment group, in compare with negative control but less than insulin as a positive control. The synergistic effect of Curcumin and insulin was lower in comparison with insulin as a positive control.
Conclusion: Curcumin could increase the gene expression but synergistic effects of Curcumin and insulin is more powerful than Insulin which can be due to the competitive action of insulin and Curcumin in activation of gene expression pathway.
