en تخصصي Special پژوهشي Research Objective: This study examined the effect of high intensity interval training (HIIT) on HNF-4&alpha; gene expression, glucose and insulin in liver tissue of type 2 diabetic male Wistar rats.<br> Materials and Methods: In this study, 20 male Wistar rats weighing 220 (&plusmn;20) grams were selected. After type 2 diabetes (T2D) induction, the samples were divided into two groups of HIIT and control. The training program was 12 weeks and 5 times a week. Fasting glucose, serum insulin and HNF-4&alpha; gene expression in the liver tissue were measured in both groups after the last exercise session by using independent T-test.<br> Results: The results showed that HIIT decreased significantly the fasting glucose (P-value: 0.001) and increased serum insulin (P-value: 0.001). Also, the expression of HNF-4&alpha; after HIIT significantly decreased in comparison to the control group (P-value: 0.003).<br> Conclusion: Based on the findings, HIIT resulted in a significant decrease in fasting glucose and a significant increase in insulin that is likely to reduce the liver HNF-4&alpha; gene expression of T2D male Wistar rats by increasing serum insulin.<br> <br> &nbsp; High intensity intermittent exercise, HNF-4α gene expression, Type 2 diabetic, Wistar rat 210 215 http://ijdo.ssu.ac.ir/browse.php?a_code=A-10-30-220&slc_lang=en&sid=1 Elham Yadegari 0000-0002-8466-0182 No PhD Student, Department of Exercise Physiology, South Tehran Branch, Islamic Azad University, Tehran, Iran. Abdol Ali Banaeifar 0000-0003-1749-6383 Yes PhD, Department of Exercise Physiology, South Tehran Branch, Islamic Azad University, Tehran, Iran Mohamad Ali Azarbayjani 0000-0002-3502-7487 No PhD, Department of Exercise Physiology, Central Tehran Branch, Islamic Azad University, Tehran, Iran Sajad Arshadi 0000-0003-0935-3673 No PhD, Department of Exercise Physiology, South Tehran Branch, Islamic Azad University, Tehran, Iran