en تخصصي Special پژوهشي Research <strong>Objective:</strong> The study aimed to determine the effect of eight weeks high intensity interval (HIIT) and sub-maximal continuous trainings on plasma betatrophin level in diabetic rats treated with metformin.<br> <strong>Materials and Methods</strong><strong>:</strong> In this experimental study, 42 diabetic wistar rats were divided into six groups (n=7): diabetic control (C), diabetic control + metformin consumption(C+M), diabetic HIIT, diabetic HIIT + metformin (HIIT+M), diabetic sub-maximal continuous training (SMCT), and diabetic sub-maximal continuous training + metformin (SMCT+M). Metformin was given 150 mg/kg/day by gavage every day, 48 hours after the end of the last training session, the rats were sacrificed. Then blood glucose and glycated hemoglobin (HbA₁c) levels were measured. One-way ANOVA test was used for statistical analysis of data.<br> <strong>Results:</strong> The level of plasma betatrophin was significantly different in the HIIT (<em>P</em>-value= 0.01) and C+M (<em>P</em>-value= 0.001) groups compared to C group. Blood glucose was significantly decreased in all training groups with or without betatrophin compared with the diabetic control group (<em>P</em>-value= 0.001). However, there were no significant changes between glucose levels in HIIT, HIIT+M, SMCT, and SMCT+M groups but SMCT showed most reduction in blood glucose.<br> <strong>Conclusion</strong><strong>:</strong> Treatment with metformin did not change&nbsp;blood glucose but two types of exercise training with high and moderate intensity reduced blood glucose thus exercise can be a good alternative modality rather than taking medicine. Interval and continuous exercise, Betatrophin, Blood glucose, Diabetes 188 192 http://ijdo.ssu.ac.ir/browse.php?a_code=A-10-30-249&slc_lang=en&sid=1 Yadollah Mahmoudi 0000-0002-2812-4422 No Ph.D. Student, Department of exercise Physiology, Faculty of Humanities and Social Sciences, Islamic Azad University, Science and Research Branch, Tehran, Iran Mandana Gholami 0000-0001-7366-5167 Yes . Assistant Professor, Department of Physical Education and Sport Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran Hojatolah Nikbakht 0000-0002-4016-8284 No Associate Professor, Department of Sport Physiology, Faculty of Humanities and Social Sciences, Islamic Azad University, Science and Research Branch, Tehran, Iran Khosrow Ebrahim 0000-0003-3250-9058 No Professor, Department of Sport Physiology, Faculty of Humanities and Social Sciences, Islamic Azad University, Science and Research Branch, Tehran, Iran Salar Bakhtiyari 0000-0002-2500-269X No Associate Professor, Department of Clinical Biochemistry, Faculty of Basic Sciences, Ilam University of Medical Sciences, Iran