en تخصصي Special پژوهشي Research <strong>Objective:</strong> The use of non-enzymatic antioxidants in food supplements and proper exercise can have a positive effect on decreasing oxidative stress by free radical hunting. The purpose of this study was to investigate the effect of aerobic training and L-carnitine supplementation on some of the oxidative stress factors in the liver of diabetic rats.<br> <strong>Materials and Methods</strong><strong>:</strong> In this experimental study, 45 male wistar rats (200-300 gr) were randomly divided into six groups: 1) sham group, 2) healthy control group 3) diabetic control group, 4) diabetic group receiving L-carnitine, 5) diabetic group of aerobic training, 6) diabetic group of aerobic training and receiving L-carnitine. The aerobic exercise protocol included six weeks, five sessions per week on the treadmill. After intervention, malondialdehyde (MDA), superoxide&nbsp;dismutase (SOD), and glutathione peroxidase (GPX) levels were determined in liver tissue.<br> <strong>Results:</strong> Six weeks of aerobic exercise had a significant effect on MDA factor in hepatic tissue in diabetic rats (<em>P</em>-value: 0.024). However, supplementation (<em>P</em>-value: 0.868) and combined intervention of aerobic exercise and supplementation of L-carnitine (<em>P</em>-value: 0.465) did not have the significant effect on MDA factor. Also, 6 weeks of aerobic training, supplementation of L-carnitine, and combined intervention had no significant effect on SOD and GPX factors of hepatic tissue in diabetic rats (<em>P</em>-value> 0.05).<br> <strong>Conclusion</strong><strong>:</strong> L-carnitine supplementation with regular exercise can have beneficial effects on hepatic antioxidant defense system in rats with type 2 diabetes. Aerobic training, L-carnitine, Antioxidant enzymes, Oxidative stress 22 29 http://ijdo.ssu.ac.ir/browse.php?a_code=A-10-30-267&slc_lang=en&sid=1 Seyed Esmaeil Jabbari 0000-0002-0824-1074 No Ph.D Student, Department of Physical Education and Sport Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran. Mandana Gholami 0000-0001-8960-4123 Yes Ph.D Department of Physical Education and Sport Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran. Hojatollah Nikbakht 0000-0002-6092-389X No Ph.D Department of Physical Education and Sport Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran. Nader Shakeri 0000-0002-7315-1382 No Ph.D Department of Physical Education and Sport Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran. Farshad Ghazalian 0000-0002-7207-9681 No Ph.D Department of Physical Education and Sport Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran.