en تخصصي Special پژوهشي Research <strong>Objective:</strong> The aim of this study was to investigate the effect of 6 weeks of detraining after 12 weeks of high intensity interval training (HIIT) on the expression of AKT1 and mTORc1 genes in the left ventricle of wistar diabetic rats.<br> <strong>Materials and Methods</strong><strong>:</strong> Twenty-eight wistar male rats were selected as the study sample and were divided in four groups of healthy control, diabetic control, diabetic HIIT and diabetic HIIT + detraining. The HIIT period was 12 weeks and the detraining period was 6 weeks. Each session consisted of 30 minutes, which included running on a treadmill with one-minute repetitions and a two-minute active recovery between them. To measure AKT1 mRNA and mTORc1 mRNA by RT-Real time PCR, a single-step single step SYBR TAKARA kits from Takara Company was used according to the company&#39;s instruction.<br> <strong>Results:</strong> HIIT caused a significant increase in AKT1 gene expression (<em>P</em>-value= 0.001). AKT1 decreased with detraining that was not significant (<em>P</em>-value= 0.34) but it was still significantly higher than before training (<em>P</em>-value= 0.017). HIIT caused a significant increase in mTORc1 gene expression (<em>P</em>-value= 0.001) and although it decreased with detraining (<em>P</em>-value= 0.15) and it was no significantly higher than before training (<em>P</em>-value= 0.19).<br> <strong>Conclusion</strong><strong>:</strong> HIIT led to increased expression of AKT1 and mTORc1 genes in type 2 diabetic rats, while also producing favorable changes in the cardiac structure of these rats. Also, 6 weeks of detraining did somewhat reduce these favorable changes. High intensity interval training, Detraining, AKT1, mTORc1, Diabetes 37 46 http://ijdo.ssu.ac.ir/browse.php?a_code=A-10-30-269&slc_lang=en&sid=1 Farzaneh Soltanipour jounaghani 0000-0001-6805-6332 No PhD candidate, Department of Exercise Physiology, Islamic Azad University, Central Tehran branch, Iran. Maghsoud Peeri 0000-0003-1415-7319 Yes Professor, Department of Exercise Physiology, Islamic Azad University, Central Tehran Branch, Iran. Mohammad-Ali Azarbayjani 0000-0002-3502-7487 No Professor, Department of Exercise Physiology, Islamic Azad University, Central Tehran Branch, Iran.