en تخصصي Special پژوهشي Research <strong>Objective:</strong> Type 2 diabetes mellitus (T2DM) is one of the most common chronic diseases<strong><em><span dir="RTL">.</span></em></strong> The CYP450 plays an important role in the biosynthesis of steroid hormones and the hormonal activity is mediated by the androgen receptor (AR) and the enzyme 5-alpha reductase (5&alpha;R). Therefore, this study aimed to investigate the relationship between these factors in T2DM.<br> <strong>Materials and Methods</strong><strong>:</strong> This case-control study was performed with 60 volunteers, including 30 diabetics and 30 healthy individuals. Demographic information of individuals was recorded and levels of CYP450, 5&alpha;R, and AR were measured in serum by ELISA. Data were analyzed by SPSS v.26 version and the significance level was less than 5%.<strong><em><span dir="RTL"></span></em></strong><br> <strong>Results:</strong> There were no significant difference &nbsp;between diabetics and healthy individuals in gender (<em>P</em>= 1), body mass index (<em>P</em>= 0.199), diastolic pressure(<em>P</em>= 0.466), uric acid(<em>P</em>= 0.202), creatinine(<em>P</em>= 0.627), low-density lipoprotein (<em>P</em>= 0.572), high-density lipoprotein(<em>P</em>=0.692); But there was a significant difference in systolic pressure(<em>P</em>= 0.034), triglyceride(<em>P</em>= 0.0001), and insulin(<em>P</em>= 0.003), between diabetics and healthy individuals. The distribution of CYP450, 5&alpha;R and AR in two groups shows that the level of all three factors is higher in diabetic people (<em>P</em>= 0.0001). Also, glycosylated hemoglobin and insulin have a direct relationship with CYP450 (<em>P</em>= 0.0001, R=0.494; <em>P</em>= 0.043, R=0.263), 5&alpha;R (<em>P</em>= 0.0001, R=0.808; <em>P</em>= 0.016, R=0.309) and with AR (<em>P</em>= 0.0001, R=0.836; <em>P</em>= 0.011, R=0.326).<br> <strong>Conclusion</strong><strong>:</strong> These results showed that there was a relationship between the levels of CYP450, 5&alpha;R, and ARs with T2DM which may explain hormonal changes in diabetic people and the different responses to treatment. Diabetes mellitus, Cytochrome P450, 5-alpha reductase, Androgen receptor 20 28 http://ijdo.ssu.ac.ir/browse.php?a_code=A-10-30-335&slc_lang=en&sid=1 Mustafa Ali Hasan Aljanabi 0000-0002-7181-0444 No Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran. Masoud Homayouni Tabrizi 0000-0002-5078-9973 No Department of Biochemistry, Faculty of Medicine, Mashhad Medical Sciences, Islamic Azad University, Mashhad, Iran. Vahid Pouresmaeil 0000-0003-1023-1032 Yes Department of Biochemistry, Faculty of Medicine, Mashhad Medical Sciences, Islamic Azad University, Mashhad, Iran.Innovative Medical Research Center, Faculty of Medicine, Mashhad Medical Sciences, Islamic Azad University, Mashhad, Iran.