en تخصصي Special پژوهشي Research <span style="font-size:11pt"><span style="text-justify:inter-ideograph"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">Objective:</span></span></span></b><b> </b><span lang="EN" style="font-size:10.5pt"><span new="" roman="" style="font-family:" times="">Hepatic glucose release plays a potential role in hyperglycemia in type 2 diabetes (T2D) patients.</span></span> <span lang="EN" style="font-size:10.5pt"><span new="" roman="" style="font-family:" times="">The aim of this experimental study was to determine the effect of 6 weeks of high-intensity interval training (HIIT) on fasting levels of glucose and insulin as well as </span></span><span style="font-size:10.5pt"><span new="" roman="" style="font-family:" times="">glucokinase </span></span><span lang="EN" style="font-size:10.5pt"><span new="" roman="" style="font-family:" times="">(GCK)</span></span> <span lang="EN" style="font-size:10.5pt"><span new="" roman="" style="font-family:" times="">expression in liver tissue in obese T2D rats.</span></span><b><span style="font-size:10.5pt"><span style="font-family:"Times New Roman","serif""></span></span></b></span></span></span></span><br> <span style="font-size:11pt"><span style="text-justify:inter-ideograph"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">Materials and Methods</span></span></span></b><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">:</span></span></span></b> <span lang="EN" style="font-size:10.5pt"><span new="" roman="" style="font-family:" times="">T2D was</span></span><span style="font-size:10.5pt"><span new="" roman="" style="font-family:" times=""> induced by a </span></span><span lang="EN" style="font-size:10.5pt"><span new="" roman="" style="font-family:" times="">high-fat diet (HFD) and streptozotocin (STZ) intraperitoneal injection in 14 male wistar rats, then were randomly divided into HIIT (n=7) and control (n=7) groups. The HIIT group practiced 6-week HIIT (5 days/ weekly). Finally, 48 hours after the last session, fasting levels of glucose, insulin, and GCK expression in liver hepatocytes of both groups were measured and compared by independent T-test </span></span><span style="font-size:10.5pt"><span new="" roman="" style="font-family:" times="">(SPSS, Version 22.0)</span></span><span lang="EN" style="font-size:10.5pt"><span new="" roman="" style="font-family:" times="">.</span></span><b><i><span dir="RTL" style="font-size:10.5pt"><span style="font-family:"Times New Roman","serif""></span></span></i></b></span></span></span></span><br> <span style="font-size:11pt"><span style="text-justify:inter-ideograph"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><b><span lang="EN" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Results:</span></span></b> <span lang="EN" style="font-size:10.5pt"><span new="" roman="" style="font-family:" times="">HIIT resulted in a significant decrease of fasting glucose compared to the control group </span></span><span style="font-size:10.5pt"><span new="" roman="" style="font-family:" times="">(<i>P</i>< 0.0001)</span></span><span lang="EN" style="font-size:10.5pt"><span new="" roman="" style="font-family:" times="">. Compared with the control group, serum insulin </span></span><span style="font-size:10.5pt"><span new="" roman="" style="font-family:" times="">(<i>P</i></span></span><span style="font-size:10.5pt"><span new="" roman="" style="font-family:" times="">: 0.018) </span></span><span lang="EN" style="font-size:10.5pt"><span new="" roman="" style="font-family:" times="">and GCK expression in hepatocytes </span></span><span style="font-size:10.5pt"><span new="" roman="" style="font-family:" times="">(<i>P</i></span></span><span style="font-size:10.5pt"><span new="" roman="" style="font-family:" times="">: 0.030)</span></span><span lang="EN" style="font-size:10.5pt"><span new="" roman="" style="font-family:" times=""> were significantly increased.</span></span><span style="font-size:10.5pt"><span style="font-family:"Times New Roman","serif""></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="text-justify:inter-ideograph"><span style="line-height:normal"><span sans-serif="" style="font-family:Calibri,"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">Conclusion</span></span></span></b><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">:</span></span></span></b> <span lang="EN" style="font-size:10.5pt"><span new="" roman="" style="font-family:" times="">Based on these findings, the improvement in glucose in response to HIIT may be rooted in increased insulin and </span></span><span style="font-size:10.5pt"><span new="" roman="" style="font-family:" times="">GCK</span></span><span lang="EN" style="font-size:10.5pt"><span new="" roman="" style="font-family:" times=""> expression in hepatocytes. However, understanding the mechanisms responsible for the effect of exercise </span></span><span style="font-size:10.5pt"><span new="" roman="" style="font-family:" times="">training</span></span><span lang="EN" style="font-size:10.5pt"><span new="" roman="" style="font-family:" times=""> on the processes affecting hepatic glucose release</span></span> <span lang="EN" style="font-size:10.5pt"><span new="" roman="" style="font-family:" times="">requires further studies.</span></span><b><i><span dir="RTL" style="font-size:10.5pt"><span style="font-family:"Times New Roman","serif""></span></span></i></b></span></span></span></span><br> &nbsp; Glucokinase expression, Hepatocyte, Interval training, Insulin 105 109 http://ijdo.ssu.ac.ir/browse.php?a_code=A-10-30-346&slc_lang=en&sid=1 Fatemeh Nikseresht 0000-0003-4492-4160 No PhD Student of Exercise Physiology, Sport Sciences Department, Lorestan University, Khorramabad, Iran. Mostafa Bahrami 0000-0002-2315-1894 Yes Assistant Professor of Exercise Physiology, Sport Sciences Department, Faculty of Literature and Human Science, Lorestan University, Khorramabad, Iran. Masoud Rahmati 0000-0003-4792-027X No Associated Professor of Exercise Physiology, Sport Sciences Department, Faculty of Literature and Human Science, Lorestan University, Khorramabad, Iran.