en تخصصي Special پژوهشي Research <span style="font-size:11pt"><span style="text-justify:inter-ideograph"><span style="line-height:115%"><span calibri="" style="font-family:"><b><span style="font-size:12.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">Objective</span></span></span></b><b><span lang="EN" style="font-size:12.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">:</span></span></span></b> <span style="font-size:10.5pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">Genetic studies indicate that available insulin influences the activity or expression of hepatic gluconeogenic genes.</span></span></span><span lang="EN" style="font-size:10.5pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""> This study aimed to assess the effect of resistance exercise program on gluconeogenic genes expression and their change relation with insulin in response to training</span></span></span><span style="font-size:10.5pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">.</span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="text-justify:inter-ideograph"><span style="line-height:115%"><span calibri="" style="font-family:"><b><span style="font-size:12.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">Materials and Methods:</span></span></span></b> <span lang="EN" style="font-size:10.5pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">18 male wistar rats by 6 weeks high-fat diet</span></span></span> <span style="font-size:10.5pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">were studied.</span></span></span><span lang="EN" style="font-size:10.5pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""> Type 2 diabetes was induced by intraperitoneal injection of STZ (25 mg/dl) in 14 rats. Finally, the studied rats were selected into </span></span></span><span style="font-size:10.5pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">3 </span></span></span><span lang="EN" style="font-size:10.5pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">groups: 1) non-diabetic, 2) control diabetic, 3) resistance diabetic. Rats in the resistance group participated in a 6-week resistance training in the form of climbing a step ladder with resistance and other groups remained none-training. ANOVA statistical test used to compare glucose, insulin and hepatic expression of </span></span></span><i><span style="font-size:10.5pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">PEPCK</span></span></span></i><span style="font-size:10.5pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">, <i>G6Pase</i></span></span></span><span lang="EN" style="font-size:10.5pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""> and <i>HNF4&alpha;</i> between groups. Correlation between insulin changes and gene expression was determined by Spearman&#39;s correlation test</span></span></span><span style="font-size:10.5pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">.</span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="text-justify:inter-ideograph"><span style="line-height:115%"><span calibri="" style="font-family:"><b><span style="font-size:12.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">Results:</span></span></span></b> <span style="font-size:10.5pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">Serum insulin significantly increased following resistance training intervention</span></span></span><span lang="EN" style="font-size:10.5pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""> (<i>P</i>= 0.043)</span></span></span><span style="font-size:10.5pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">. </span></span></span><span lang="EN" style="font-size:10.5pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">A significant decrease was also observed in f</span></span></span><span style="font-size:10.5pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">asting glucose </span></span></span><span lang="EN" style="font-size:10.5pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">(<i>P</i>= 0.001)</span></span></span><span style="font-size:10.5pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""> and </span></span></span><span lang="EN" style="font-size:10.5pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">hepatic expression of </span></span></span><i><span style="font-size:10.5pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">PEPCK</span></span></span></i> <span lang="EN" style="font-size:10.5pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">(<i>P</i>= 0.001)</span></span></span><span style="font-size:10.5pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">, <i>G6Pase</i> </span></span></span><span lang="EN" style="font-size:10.5pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">(<i>P</i>= 0.001) and <i>HNF4&alpha;</i> (<i>P</i>= 0.011) compared to control diabetes rats. Significant inverse correlation was observed in the change of insulin with <i>HNF4&alpha;</i> (<i>P</i>= 0.001), <i>PEPCK</i> (<i>P</i>= 0.013) and </span></span></span><i><span style="font-size:10.5pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">G6Pase</span></span></span></i><span lang="EN" style="font-size:10.5pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""> (<i>P</i>= 0.043) in response to resistance training</span></span></span><span style="font-size:10.5pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">.</span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="text-justify:inter-ideograph"><span style="line-height:115%"><span calibri="" style="font-family:"><b><span lang="EN" style="font-size:12.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">Conclusion:</span></span></span></b><span lang="EN" style="font-size:10.5pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""> Resistance training is associated with changes in the expression of gluconeogenic genes, and these changes can probably be attributed to changes in serum insulin</span></span></span><span style="font-size:10.5pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">.</span></span></span><b><span lang="EN" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"Times New Roman","serif""></span></span></span></b></span></span></span></span><br> &nbsp; Diabetes, Resistance Training, Gluconeogenic genes, Insulin 40 47 http://ijdo.ssu.ac.ir/browse.php?a_code=A-10-30-437&slc_lang=en&sid=1 Mojtaba Eizadi 0000-0003-1989-692X Yes Assistant Professor of Exercise Physiology, Saveh Branch, Islamic Azad University, Saveh, Iran. Sayed Sadegh Salehi No PHD Candidate, Department of Exercise physiology, Islamshahr Branch, Islamic Azad University, Tehran, Iran. Mohammad Rashidi No Assistant Professor of Exercise Physiology, Semnan Branch, Islamic Azad University, Semnan, Iran.