en تخصصي Special پژوهشي Research <span style="font-size:11pt"><span style="text-justify:inter-ideograph"><span style="line-height:115%"><span calibri="" style="font-family:"><b><span style="font-size:10.5pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">Objective: </span></span></span></b><i><span style="font-size:10.5pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">SIRT1</span></span></span></i><span style="font-size:10.5pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""> is a key regulator of adipose tissue&#39;s energy balance, inflammation, and mitochondrial function. This study was designed to investigate the effect of different training modalities</span></span></span><span lang="EN-GB" style="font-size:10.5pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">,</span></span></span><span style="font-size:10.5pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""> Moderate-Intensity Aerobic Training (MIAT), High-Intensity Aerobic Training (HIAT), and High-Intensity Interval Training (HIIT), on <i>SIRT1</i> gene expression in subcutaneous adipose tissue of male Wistar rats.</span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="text-justify:inter-ideograph"><span style="line-height:115%"><span calibri="" style="font-family:"><b><span style="font-size:10.5pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">Materials and Methods: </span></span></span></b><span style="font-size:10.5pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">Thirty-two 8-week-old male Wistar rats (237 &plusmn; 33 g) were randomly assigned to four groups (n= 8): Control, MIAT, HIAT, and HIIT. Animals were housed under controlled conditions and had free access to food and water. Training protocols were performed five times per week for eight weeks. After 12 hours of fasting and 24 hours post-intervention, subcutaneous abdominal adipose tissue was collected. <i>SIRT1</i> gene expression was analyzed using RT-PCR. Data were statistically evaluated using one-way ANOVA and LSD post hoc test (<i>P</i>< 0.05) in SPSS-22.</span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="text-justify:inter-ideograph"><span style="line-height:115%"><span calibri="" style="font-family:"><b><span style="font-size:10.5pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">Results: </span></span></span></b><span style="font-size:10.5pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">The results showed that all three training models - MIAT (<i>P</i>= 0.023), HIAT (<i>P</i>= 0.001), and HIIT (<i>P</i>= 0.003) - significantly increased <i>SIRT1</i> gene expression compared </span></span></span><span lang="EN-GB" style="font-size:10.5pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">to</span></span></span><span style="font-size:10.5pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""> the control group.</span></span></span><b> </b></span></span></span></span><br> <span style="font-size:11pt"><span style="text-justify:inter-ideograph"><span style="line-height:115%"><span calibri="" style="font-family:"><b><span style="font-size:10.5pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">Conclusion:</span></span></span></b><span style="font-size:10.5pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""> Although all training modalities significantly increased <i>SIRT1</i> gene expression compared to the control group, the HIAT group showed the highest mean expression level. However, no statistically significant difference was observed between the <a name="_Hlk210459384">training</a> groups.</span></span></span><span lang="EN-GB" style="font-size:10.5pt"><span style="line-height:115%"><span style="font-family:"Times New Roman",serif"></span></span></span></span></span></span></span><br> &nbsp; Aerobic exercise training, Adipose tissue, Gene expression, SIRT1 248 255 http://ijdo.ssu.ac.ir/browse.php?a_code=A-10-30-462&slc_lang=en&sid=1 Amirhossein Tavasoli 0009-0006-9206-9903 No Master of Science in Applied Exercise Physiology, Payam Noor University, Karaj Center, Karaj, Iran. Mohammad Shariatzadeh Joneydi 0000-0002-8492-2875 No Associate Professor of Exercise Physiology, Physical Education and Sport Sciences Research Institute, Tehran, Iran. Javad Ramezani 0000-0002-1980-5605 Yes Assistant Professor, Department of Sport Sciences, Payam Noor University, Tehran, Iran.