Iranian Journal of Diabetes and Obesity

en Anti-Hyperglycemic Role of Hydroethanolic Extract of Foeniculum Vulgare Seed on Diabetic Wistar Albino Rats تخصصي Special پژوهشي Research Objective: Foeniculum vulgare has been suggested as a potential option for the management of hyperglycemia. This study aimed to evaluate the anti-hyperglycemic effects of the hydro-ethanolic extract of Foeniculum vulgare seeds (HEEFVS) in alloxan-induced diabetic rats. Materials and Methods: Thirty-six male rats were randomly divided into six groups (I–VI). Diabetes was induced by intraperitoneal injection of alloxan (175 mg/kg) in groups II–VI. Groups I and II received distilled water; groups III, IV, and V were treated with HEEFVS at doses of 100, 200, and 400 mg/kg body weight/day, respectively; and group VI received glibenclamide (5 mg/kg body weight/day). Serum glucose, total cholesterol (TC), triglycerides (TG), aspartate transaminase (AST), alanine transaminase (ALT), and histological analysis of the liver were assessed. Results: Administration of HEEFVS at 200 and 400 mg/kg significantly decreased ALT and AST activities (P< 0.001). Total cholesterol levels were significantly reduced (P< 0.001) at all tested doses (100, 200, and 400 mg/kg). Triglyceride levels showed a significant reduction (P< 0.01) only at the 400 mg/kg dose. During the second and third weeks, administration of 200 and 400 mg/kg markedly reduced glucose levels (P< 0.001). Complete regeneration of liver tissue was observed in the 400 mg/kg group, while partial restoration was seen in the 100 mg/kg and 200 mg/kg groups. DPPH radical scavenging and α-amylase inhibitory activities of HEEFVS demonstrated IC50 values of 146.6 µg/ml and 8.47 µg/ml, respectively. The LD50 of HEEFVS was greater than 3500 mg/kg. Conclusion: HEEFVS exhibits strong hepato-protective, hypolipidemic and anti-hyperglycemic effects.   Alloxan, Diabetes mellitus, Wistar albino rats, Foeniculum vulgare, Anti-hyperglycemic effect 256 265 http://ijdo.ssu.ac.ir/browse.php?a_code=A-10-30-463&slc_lang=en&sid=1 Feven Tigstu Ayele : 0009-0000-3157-331x Yes Department of Biomedical, Dire Dawa University, Dire Dawa, Ethiopia. Winner Kucha Dewelle 0000-0002-3083-2022 No Department of Clinical Chemistry, Haramaya University, Harar, Ethiopia. Maria Degef Teklemariam 0000-0002-1235-5971 No Department of Biochemistry, Addis Ababa University, Addis Ababa, Ethiopia. Gnanasekaran Natesan 0000-0002-1133-5602 No Department of Biochemistry, Addis Ababa University, Addis Ababa, Ethiopia. Yonas Bekuretsion Abraham 0000-0001-8717-3411 No Department of Pathology, Addis Ababa University, Addis Ababa, Ethiopia. Gelila Woredebrhan Mesfin 0009-0002-9328-8113 No Biomedical Department, Dilla University, Dilla, Ethiopia. Chala Bashea Hailu 0000-0002-5804-2153 No National Clinical Chemistry Reference Laboratory, Ethiopian Public Health Institute, Addis Ababa, Ethiopia. Solomon Genet Gebre 0000-0002-5433-0107 No Department of Biochemistry, Addis Ababa University, Addis Ababa, Ethiopia.