Volume 13, Issue 3 (volume 13, number 3 2021)                   IJDO 2021, 13(3): 173-177 | Back to browse issues page

XML Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Bostani M, Noaein S A. The Effect of Continuous Aerobic Training on Bax/Bcl2 Ratio in Pancreatic Tissue Diabetic Rats. IJDO. 2021; 13 (3) :173-177
URL: http://ijdo.ssu.ac.ir/article-1-649-en.html
Department of Physical Education, Ahvaz Branch, Islamic Azad University, Ahvaz, Iran.
Abstract:   (316 Views)
Objective: Apoptosis of pancreatic beta cells plays an important role in the pathogenesis of type 1 diabetes. the purpose of this study was to investigate the effect of continuous aerobic training on Bax/Bcl-2 ratio in pancreatic tissue of streptozotocin (STZ)-induced diabetic rats.
Materials and Methods: A total number of 40 male Wistar rats, were divided into healthy control (HC), healthy trained (HT), diabetic control (DC), and diabetic trained (DT) groups. Diabetes was also induced by a single intraperitoneally injection of STZ (45 mg/kg). the training groups performed the exercise on the treadmill for five consecutive days within six weeks with 10-18 m/min intensity and 10-30 minute duration based on the principle of overload. the pancreatic tissue levels of the Bax and the Bcl-2 proteins were further determined via the ELISA method. The analysis of variance test (ANOVA) with Tukey's post hoc test was used for analyzing the data.
Results: The results showed that the induction of diabetes had significantly decreased the levels of Bcl-2 protein and increased the levels of Bax protein and Bax/Bcl-2 ratio in the pancreatic tissue (P-value=0.001,Effect Size=0.29). As well, the findings showed that six weeks of aerobic exercise training had significantly increased the levels of Bcl-2 and significantly decreased the levels of Bax and Bax/Bcl-2 ratio in the DT group (P-value=0.012, Effect Size=0.23).
Conclusion: According to the results of this study, exercise can be considered an effective strategy to reduce the rate of diabetic-induced apoptosis and control its complications.
Full-Text [PDF 451 kb]   (130 Downloads)    
Type of Study: Research | Subject: Special
Received: 2021/09/14 | Accepted: 2021/09/19 | Published: 2021/09/19

1. Atkinson MA. Thirty years of investigating the autoimmune basis for type 1 diabetes: why can't we prevent or reverse this disease?. Diabetes. 2005;54(5):1253-63. [DOI:10.2337/diabetes.54.5.1253]
2. Kim KA, Lee MS. Recent progress in research on beta-cell apoptosis by cytokines. Front Biosci. 2009;14:657-64. [DOI:10.2741/3271]
3. Bauer D, Werth F, Nguyen HA, Kiecker F, Eberle J. Critical role of reactive oxygen species (ROS) for synergistic enhancement of apoptosis by vemurafenib and the potassium channel inhibitor TRAM-34 in melanoma cells. Cell death & disease. 2017;8(2):e2594-. [DOI:10.1038/cddis.2017.6]
4. Youle RJ, Strasser A. The BCL-2 protein family: opposing activities that mediate cell death. Nature reviews Molecular cell biology. 2008;9(1):47-59. [DOI:10.1038/nrm2308]
5. Costello JL, Castro IG, Camões F, Schrader TA, McNeall D, Yang J, et al. Predicting the targeting of tail-anchored proteins to subcellular compartments in mammalian cells. Journal of cell science. 2017;130(9):1675-87. [DOI:10.1242/jcs.200204]
6. Nayki U, Onk D, Balci G, Nayki C, Onk A, Gunay M. The effects of diabetes mellitus on ovarian injury and reserve: an experimental study. Gynecologic and obstetric investigation. 2016;81(5):424-9. [DOI:10.1159/000442287]
7. Cerqueira É, Marinho DA, Neiva HP, Lourenço O. Inflammatory effects of high and moderate intensity exercise-A systematic review. Frontiers in physiology. 2020;10:1550. [DOI:10.3389/fphys.2019.01550]
8. Gomez-Cabrera MC, Viña J, Ji LL. Interplay of oxidants and antioxidants during exercise: implications for muscle health. The Physician and sportsmedicine. 2009;37(4):116-23. [DOI:10.3810/psm.2009.12.1749]
9. Batista Jr ML, Rosa JC, Lopes RD, Lira FS, Martins Jr E, Yamashita AS, et al. Exercise training changes IL-10/TNF-α ratio in the skeletal muscle of post-MI rats. Cytokine. 2010;49(1):102-8. [DOI:10.1016/j.cyto.2009.10.007]
10. No MH, Heo JW, Yoo SZ, Kim CJ, Park DH, Kang JH, et al. Effects of aging and exercise training on mitochondrial function and apoptosis in the rat heart. Pflügers Archiv-European Journal of Physiology. 2020;472(2):179-93. [DOI:10.1007/s00424-020-02357-6]
11. Fisher-Wellman K, Bloomer RJ. Acute exercise and oxidative stress: a 30 year history. Dynamic medicine. 2009;8(1):1-25. [DOI:10.1186/1476-5918-8-1]
12. Dejean LM, Martinez-Caballero S, Manon S, Kinnally KW. Regulation of the mitochondrial apoptosis-induced channel, MAC, by BCL-2 family proteins. Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease. 2006;1762(2):191-201. [DOI:10.1016/j.bbadis.2005.07.002]
13. Peterson JM, Bryner RW, Sindler A, Frisbee JC, Alway SE. Mitochondrial apoptotic signaling is elevated in cardiac but not skeletal muscle in the obese Zucker rat and is reduced with aerobic exercise. Journal of applied physiology. 2008;105(6):1934-43. [DOI:10.1152/japplphysiol.00037.2008]
14. Fang J, Wu L, Chen L. Postconditioning attenuates cardiocyte ultrastructure injury and apoptosis by blocking mitochondrial permeability transition in rats. Acta cardiologica. 2008;63(3):377-87. [DOI:10.2143/AC.63.3.1020316]
15. Garcia-Saez AJ. The secrets of the Bcl-2 family. Cell Death & Differentiation. 2012;19(11):1733-40. [DOI:10.1038/cdd.2012.105]
16. Kwak HB. Effects of aging and exercise training on apoptosis in the heart. Journal of exercise rehabilitation. 2013;9(2):212. [DOI:10.12965/jer.130002]
17. Vainshtein A, Kazak L, Hood DA. Effects of endurance training on apoptotic susceptibility in striated muscle. Journal of applied physiology. 2011;110(6):1638-45. [DOI:10.1152/japplphysiol.00020.2011]

Add your comments about this article : Your username or Email:

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2022 CC BY-NC 4.0 | Iranian Journal of Diabetes and Obesity

Designed & Developed by : Yektaweb