Iranian Journal of Diabetes and Obesity
Shahid Sadoughi University Of Medical Sciences
Mon, Dec 30, 2024
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Volume 12, Issue 4 (12-2020)
IJDO 2020, 12(4): 233-240 |
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Malekpour-Dehkordi Z, Mohiti-Ardekani J, Naghiaee Y, Teimourian S, Hemati M, Nourbakhsh M. Metformin and Pioglitazone Reduce Gene Expression of Inflammatory Factors in Insulin Resistant and Hypertrophied Adipocytes. IJDO 2020; 12 (4) :233-240
URL: http://ijdo.ssu.ac.ir/article-1-595-en.html
URL: http://ijdo.ssu.ac.ir/article-1-595-en.html
Zahra Malekpour-Dehkordi 
, Javad Mohiti-Ardekani , Yousof Naghiaee , Shahram Teimourian , Mahdie Hemati , Mitra Nourbakhsh *
, Javad Mohiti-Ardekani , Yousof Naghiaee , Shahram Teimourian , Mahdie Hemati , Mitra Nourbakhsh *
Department of Biochemistry, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran
Abstract: (1120 Views)
Objective: In obesity, chronic low grade inflammation, created by induction of pro-inflammatory markers, causes adipocyte dysfunction in adipose tissue. Adipocytes dysfunction is associated with various diseases including insulin resistance and obesity. In obesity, inflammatory factors such as osteopontin (OPN), angiopoietin-like protein 2 (Angptl2) and transforming growth factor-β (TGF-β) are induced in adipose tissue. Metformin and pioglitazone that are used to modulate inflammation, but the relevant mechanism is poorly understood. This study aimed to investigate the effect of metformin and pioglitazone as anti-diabetic drugs, on gene expression of osteopontin, Angptl2 and TGF-β as inflammatory factors in insulin resistance and hypertrophied adipocyte in 3T3-L1 cell line model in vitro.
Materials and Methods: In this experimental research, we differentiated3T3-L1 preadipocytes to adipocytes. The adipocytes treated in insulin resistance and hypertrophied conditions with metformin and pioglitazone and assayed gene expression of OPN, Angptl2 and TGF-β by Real-Time PCR. Data was analyzed by SPSS statistic software.
Results: The results showed that expression of OPN, Angptl2, and TGF-β were increased significantly over 2-fold (P-value< 0.05) in insulin resistance and hypertrophied adipocytes compared to normal adipocytes. Pre- and co-treatment with metformin and pioglitazone led to reduced expression of Angptl2 and TGF-β. Only metformin significantly reduced the expression of Angptl2, TGF-β and OPN in hypertrophied adipocyte.
Conclusion: These results support the proposal that metformin and pioglitazone reduce gene expression of inflammatory factors in insulin resistant and hypertrophied adipocytes.
Materials and Methods: In this experimental research, we differentiated3T3-L1 preadipocytes to adipocytes. The adipocytes treated in insulin resistance and hypertrophied conditions with metformin and pioglitazone and assayed gene expression of OPN, Angptl2 and TGF-β by Real-Time PCR. Data was analyzed by SPSS statistic software.
Results: The results showed that expression of OPN, Angptl2, and TGF-β were increased significantly over 2-fold (P-value< 0.05) in insulin resistance and hypertrophied adipocytes compared to normal adipocytes. Pre- and co-treatment with metformin and pioglitazone led to reduced expression of Angptl2 and TGF-β. Only metformin significantly reduced the expression of Angptl2, TGF-β and OPN in hypertrophied adipocyte.
Conclusion: These results support the proposal that metformin and pioglitazone reduce gene expression of inflammatory factors in insulin resistant and hypertrophied adipocytes.
Type of Study: Research |
Subject:
Special
Received: 2021/01/12 | Accepted: 2020/12/20 | Published: 2020/12/20
Received: 2021/01/12 | Accepted: 2020/12/20 | Published: 2020/12/20
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