Iranian Journal of Diabetes and Obesity
Shahid Sadoughi University Of Medical Sciences
Tue, Nov 19, 2024
[Archive]
Volume 13, Issue 2 (6-2021)
IJDO 2021, 13(2): 82-87 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Sheikh Samani P, Shahanipour K, Noori Diziche A, Adibnejad M. The Comparison of the Protective Effect of L-Carnitine, Chromium and Vitamin D with Metformin on Liver, Antioxidant Enzyme Activity, and Iron Metabolism in Diabetic Rats. IJDO 2021; 13 (2) :82-87
URL: http://ijdo.ssu.ac.ir/article-1-629-en.html
URL: http://ijdo.ssu.ac.ir/article-1-629-en.html
Department of Biochemistry, Falavarjan Branch, Islamic Azad University, Isfahan, Iran.
Abstract: (1093 Views)
Objective: Due to the lack of information about the positive or negative effects of L-carnitine, chromium, vitamin D, and the uptake of a safe dietary supplement to reduce the effects of diabetes, it seems essential to determine the effects of these drugs on diabetes.
Materials and Methods: Wistar rats were divided into 12 groups, control, diabetic, and intact, each of which was treated with metformin, L-carnitine, vitamin D, and chromium or a combination of L-carnitine, vitamin D, and chromium. The serum levels of liver function parameters, iron, TIBC, Catalase and GPX activities were measured. Data were statistically analyzed by one-way ANOVA using SPSS 18 software. Statistical significance among the groups was determined using LSD test, and P-value< 0.05 was considered significant in all cases.
Results: AST activity in diabetic groups and those receiving chromium and L-carnitine has significantly reduced (P-value= 0.009). A significant reduction in ALT and ALP activity in the diabetic groups receiving vitamin D and combined and non-diabetic groups receiving metformin were observed, in contrast to a significant increase in ALT activity in diabetic recipients of L-carnitine (P-value= 0.009).
Conclusion: L-carnitine, chromium, and vitamin D supplements have synergistic effects and a combination of them has the best protective effect on factors that have been studied.
Materials and Methods: Wistar rats were divided into 12 groups, control, diabetic, and intact, each of which was treated with metformin, L-carnitine, vitamin D, and chromium or a combination of L-carnitine, vitamin D, and chromium. The serum levels of liver function parameters, iron, TIBC, Catalase and GPX activities were measured. Data were statistically analyzed by one-way ANOVA using SPSS 18 software. Statistical significance among the groups was determined using LSD test, and P-value< 0.05 was considered significant in all cases.
Results: AST activity in diabetic groups and those receiving chromium and L-carnitine has significantly reduced (P-value= 0.009). A significant reduction in ALT and ALP activity in the diabetic groups receiving vitamin D and combined and non-diabetic groups receiving metformin were observed, in contrast to a significant increase in ALT activity in diabetic recipients of L-carnitine (P-value= 0.009).
Conclusion: L-carnitine, chromium, and vitamin D supplements have synergistic effects and a combination of them has the best protective effect on factors that have been studied.
Type of Study: Research |
Subject:
Special
Received: 2021/06/16 | Accepted: 2021/06/20 | Published: 2021/06/20
Received: 2021/06/16 | Accepted: 2021/06/20 | Published: 2021/06/20
References
1. Alvarez JA, Ashraf A. Role of vitamin D in insulin secretion and insulin sensitivity for glucose homeostasis. International journal of endocrinology. 2010;2010. [DOI:10.1155/2010/351385]
2. Ouslimani N, Peynet J, Bonnefont-Rousselot D, Thérond P, Legrand A, Beaudeux JL. Metformin decreases intracellular production of reactive oxygen species in aortic endothelial cells. Metabolism. 2005;54(6):829-34. [DOI:10.1016/j.metabol.2005.01.029]
3. Trow L, Lewis J, Greenwood R, Sampson M, Self K, Crews H, et al. Lack of effect of dietary chromium supplementation on glucose tolerance, plasma insulin and lipoprotein levels in patients with type 2 diabetes. International journal for vitamin and nutrition research. 2000;70(1):14-8. [DOI:10.1024/0300-9831.70.1.14]
4. Noichri Y, Chalghoum A, Chkioua L, Baudin B, Ernez S, Ferchichi S, et al. Low erythrocyte catalase enzyme activity is correlated with high serum total homocysteine levels in Tunisian patients with acute myocardial infarction. Diagnostic pathology. 2013;8(1):1-7. [DOI:10.1186/1746-1596-8-68]
5. Singh A, Rangasamy T, Thimmulappa RK, Lee H, Osburn WO, Brigelius-Flohe R, et al. Erratum: Glutathione peroxidase 2, the major cigarette smoke-inducible isoform of GPX in lungs, is regulated by Nrf2 (American Journal of Respiratory Cell and Molecular Biology (2006) 35 (639-650)). American journal of respiratory cell and molecular biology. 2007;36(5):642. [DOI:10.1165/rcmb.2005-0325OC]
6. Adamcakova-Dodd A, Stebounova LV, Kim JS, Vorrink SU, Ault AP, T O'Shaughnessy P, et al. Toxicity assessment of zinc oxide nanoparticles using sub-acute and sub-chronic murine inhalation models. Particle and fibre toxicology. 2014;11(1):1-5. [DOI:10.1186/1743-8977-11-15]
7. Sharifi F, Sazandeh Sh. Serum ferritin in type 2 diabetes mellitus and Its relationship with HbA1c. Acta Medica Iranica, 2004; 42 (2): 142-5.
8. Pasaoglu H, Sancak B, Bukan N. Lipid peroxidation and resistance to oxidation in patients with type 2 diabetes mellitus. The Tohoku journal of experimental medicine. 2004;203(3):211-8. [DOI:10.1620/tjem.203.211]
9. Oyedemi SO, Bradley G, Afolayan AJ. Beneficial effect of aqueous Stem Bark Extracts of Strychnos henningsii gilg in streptozotocin-nicotinamide induced type 2 diabetic Wistar rats. International Journal of pharmacology. 2011;7(7):773-81. [DOI:10.3923/ijp.2011.773.781]
10. Kume E, Fujimura H, Matsuki N, Ito M, Aruga C, Toriumi W, et al. Hepatic changes in the acute phase of streptozotocin (SZ)-induced diabetes in mice. Experimental and Toxicologic Pathology. 2004;55(6):467-80. [DOI:10.1078/0940-2993-00351]
11. Ramesh B, Viswanathan P, Pugalendi KV. Protective effect of Umbelliferone on membranous fatty acid composition in streptozotocin-induced diabetic rats. European journal of pharmacology. 2007;566(1-3):231-9. [DOI:10.1016/j.ejphar.2007.03.045]
12. Pradhan AD, Everett BM, Cook NR, Rifai N, Ridker PM. Effects of initiating insulin and metformin on glycemic control and inflammatory biomarkers among patients with type 2 diabetes: the Lancet randomized trial. Jama. 2009;302(11):1186-94. [DOI:10.1001/jama.2009.1347]
13. Shafi Zad A, Rezaie A, Rohbani Nobar M, Mohajeri D, Rahmani J. Effect of metformin on serum glucose, lipid profiles, and oxidative stress in the alloxan-induced diabetic rats. Journal Of Comparative Pathobiology Iran. 2013;10 (1):865-872.(in Persian)
14. Yin RV, Phung OJ. Effect of chromium supplementation on glycated hemoglobin and fasting plasma glucose in patients with diabetes mellitus. Nutrition journal. 2015;14(1):1-9. [DOI:10.1186/1475-2891-14-14]
15. Cefalu WT, Hu FB. Role of chromium in human health and in diabetes. Diabetes care. 2004;27(11):2741-51. [DOI:10.2337/diacare.27.11.2741]
16. Bacurau RF, Navarro F, Bassit RA, Meneguello MO, Santos RV, Almeida AL. Does exercise training interfere with the effects of l-carnitine supplementation?. Nutrition. 2003;19(4):337-41. [DOI:10.1016/S0899-9007(02)01015-8]
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
