Effect of Black Grape Seed(Vitis Vinifera) Extract Consumption with Moderate-Intensity Aerobic Training on the Expression of MicroRNAs in Type 1 Diabetic Cardiac Tissue Rats

Shirani Bidabadi, Maryam; Banaei Borojeni, Jamshid; Keshavarz, Saeed; Karimi, Mohammad

doi:10.18502/ijdo.v13i4.8003

Volume 13, Issue 4 (12-2021) IJDO 2021, 13(4): 248-254 | Back to browse issues page

‎ 10.18502/ijdo.v13i4.8003

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Shirani Bidabadi M, Banaei Borojeni J, Keshavarz S, Karimi M. Effect of Black Grape Seed(Vitis Vinifera) Extract Consumption with Moderate-Intensity Aerobic Training on the Expression of MicroRNAs in Type 1 Diabetic Cardiac Tissue Rats. IJDO 2021; 13 (4) :248-254
URL: http://ijdo.ssu.ac.ir/article-1-669-en.html

Effect of Black Grape Seed(Vitis Vinifera) Extract Consumption with Moderate-Intensity Aerobic Training on the Expression of MicroRNAs in Type 1 Diabetic Cardiac Tissue Rats

Maryam Shirani Bidabadi

, Jamshid Banaei Borojeni ^*

, Saeed Keshavarz

, Mohammad Karimi

Sports Medicine Research Center, Najafabad Branch, Islamic Azad University, Najafabad, Iran.

Abstract: (1136 Views)

Objective: This study aimed to evaluate the effect of consuming grape seed extract with moderate-intensity aerobic training on the expression of miR-126 and miR-29 in the cardiac tissue in type 1 diabetic male rats.
Materials and Methods: 40 rats with an initial weight range of 160-220 g were divided into five groups: Training + Extract, Training, Extract, Diabetic / Control, and Healthy / Control. Aerobic training program was moderate intensity and rats performed aerobic training for 60 minutes a day with the intensity 70 to 75% of maximum oxygen consumption (28 meters per minute). Grape seed extract was also administered by gavage at a dose of 40 mg/kg per day.
Results: Expression of both miRNAs in the three groups of training + extract, healthy training and control was significantly higher than the two groups of extract and diabetic control (P-value= 0.001). The difference between the three groups of training + extract, healthy training and control and also the difference between the two groups of extract and diabetic control were not significant (P-value> 0.05).
Conclusion: Aerobic training may be able to prevent cardiac disease caused by type 1 diabetes.

Keywords: Diabetes, Aerobic training, Grape Seed, miR-126, miR-29a

Full-Text [PDF 128 kb] (413 Downloads)

Type of Study: Research | Subject: Special
Received: 2021/06/17 | Accepted: 2021/10/4 | Published: 2021/11/1

References

1. Raffort J, Hinault C, Dumortier O, Van Obberghen E. Circulating microRNAs and diabetes: potential applications in medical practice. Diabetologia. 2015;58(9):1978-92. [DOI:10.1007/s00125-015-3680-y]

2. Kantharidis P, Wang B, Carew RM, Lan HY. Diabetes complications: the microRNA perspective. Diabetes. 2011;60(7):1832-7. [DOI:10.2337/db11-0082]

3. Winbanks CE, Ooi JY, Nguyen SS, McMullen JR, Bernardo BC. Micro RNA s differentially regulated in cardiac and skeletal muscle in health and disease: Potential drug targets?. Clinical and Experimental Pharmacology and Physiology. 2014;41(9):727-37. [DOI:10.1111/1440-1681.12281]

4. Rawal S, Manning P, Katare R. Cardiovascular microRNAs: as modulators and diagnostic biomarkers of diabetic heart disease. Cardiovascular diabetology. 2014;13(1):1-24. [DOI:10.1186/1475-2840-13-44]

5. nD Jr DS, Fernandes T, Soci UP, Monteiro AW, Phillips MI, EM DO. Swimming training in rats increases cardiac MicroRNA-126 expression and angiogenesis. Medicine and science in sports and exercise. 2012;44(8):1453-62. [DOI:10.1249/MSS.0b013e31824e8a36]

6. Gupta S, Das B, Sen S. Cardiac hypertrophy: mechanisms and therapeutic opportunities. Antioxidants & redox signaling. 2007;9(6):623-52. [DOI:10.1089/ars.2007.1474]

7. Kriegel AJ, Liu Y, Fang Y, Ding X, Liang M. The miR-29 family: genomics, cell biology, and relevance to renal and cardiovascular injury. Physiological genomics. 2012;44(4):237-44. [DOI:10.1152/physiolgenomics.00141.2011]

8. Terauchi M, Horiguchi N, Kajiyama A, Akiyoshi M, Owa Y, Kato K, et al. Effects of grape seed proanthocyanidin extract on menopausal symptoms, body composition, and cardiovascular parameters in middle-aged women: a randomized, double-blind, placebo-controlled pilot study. Menopause. 2014;21(9):990-6. [DOI:10.1097/GME.0000000000000200]

9. Ferrazzano GF, Amato I, Ingenito A, Zarrelli A, Pinto G, Pollio A. Plant polyphenols and their anti-cariogenic properties: a review. Molecules. 2011;16(2):1486-507. [DOI:10.3390/molecules16021486]

10. Saada HN, Said UZ, Meky NH, Azime AS. Grape seed extract Vitis vinifera protects against radiation‐induced oxidative damage and metabolic disorders in rats. Phytotherapy Research: An International Journal Devoted to Pharmacological and Toxicological Evaluation of Natural Product Derivatives. 2009;23(3):434-8. [DOI:10.1002/ptr.2684]

11. Bugger H, Abel ED. Rodent models of diabetic cardiomyopathy. Disease models & mechanisms. 2009;2(9-10):454-66. [DOI:10.1242/dmm.001941]

12. Garekani ET, Mohebbi H, Kraemer RR, Fathi R. Exercise training intensity/volume affects plasma and tissue adiponectin concentrations in the male rat. Peptides. 2011;32(5):1008-12. [DOI:10.1016/j.peptides.2011.01.027]

13. de Blanco EC. Review of Drugs of Natural Origin. A Treatise of Pharmacognosy. J. Nat. Prod. 2016;79:1707. [DOI:10.1021/acs.jnatprod.6b00503]

14. Liu Y, Gao G, Yang C, Zhou K, Shen B, Liang H, et al. Stability of miR-126 in urine and its potential as a biomarker for renal endothelial injury with diabetic nephropathy. International journal of endocrinology. 2014;2014. [DOI:10.1155/2014/393109]

15. Nekouei A, Kordi M, Choobineh S, Soleimani M, Shafiee A, Hadidi V. The Effect Of Eight Week Continuous Training On Expression Of Micrornas29 Mrna, In Healthy Male Rat's Cardiac Muscle. 2016;17(6):113-120.(in Persian)

16. Fernandes T, Soci UP, Oliveira EM. Eccentric and concentric cardiac hypertrophy induced by exercise training: microRNAs and molecular determinants. Brazilian Journal of Medical and Biological Research. 2011;44:836-47. [DOI:10.1590/S0100-879X2011007500112]

17. Sanna RS, Muthangi S, BK CS, Devi SA. Grape seed proanthocyanidin extract and insulin prevents cognitive decline in type 1 diabetic rat by impacting Bcl-2 and Bax in the prefrontal cortex. Metabolic brain disease. 2019;34(1):103-17. [DOI:10.1007/s11011-018-0320-5]

18. Irak K, Yıldırım S, Mert H, Mert N. Grape seed extract effects on serum amylase levels and immunohistochemical alterations in Streptozotocin-induced diabetic rats. Cellular and Molecular Biology. 2018;64(4):92-7. [DOI:10.14715/cmb/2018.64.4.15]