Volume 14, Issue 1 (volume 14, number 1 2022)                   IJDO 2022, 14(1): 37-43 | Back to browse issues page

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Azizian-Farsani F, Abedpoor N, Derakhshan M, Nasr-Esfahani M H, Sheikhha M H, Ghaedi K. Protective Effects of the Combination of the Herbal Compound Against Inflammation Related to Obesity and Colitis Induced by Diet in Mice. IJDO 2022; 14 (1) :37-43
URL: http://ijdo.ssu.ac.ir/article-1-685-en.html
Department of Medical Genetics, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.Biotechnology Research center, International Campus, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Abstract:   (751 Views)
Objective: High-fat diet (HFD) rises the susceptibility of both obesity and consequently Inflammatory Bowel Disease (IBD). We designed a study to investigate the improving effects of herbal extract (HE, the combination of turmeric, ginger, boswellia, and cat’s claw extract) on the risk of high AGEs-fat diet 60% (HFD) mice induced colitis and obesity.
Materials and Methods: Four-week-old C57BL/6 male mice after 2 weeks adaptation with normal diet were fed with either HFD or normal diets. After 6 weeks of being on diet, animals received HE for 16 weeks. Obesity index markers were determined as well as histological studies using H&E (Hematoxylin-eosin) staining. Colonic expression of IL-1β was determined. Data analysis was performed by utilizing Kruskal-Wallis and Mann-Whitney test for post-hoc comparisons, and SPSS (version 17.0) and GraphPad Prism Software (Version 8.0, USA).
Results: HE decreased histological scores (by 6-fold) in HFD diet-fed mice, and reduced myeloperoxidase activity (by 2.2-fold), and ratio of colon weight to length (by 4-fold) in HFD diet-fed mice. Moreover, HE prevented intestinal permeability through the restoration of ZO-1 (by 4-fold) and immune homeostasis by modulation of IL-1β (by 2.4-fold) expression.
Conclusion: HFD induced obesity-associated colitis. HE decreased the colitis symptoms in HFD diet-fed mice, with the reduction of inflammation.
Full-Text [PDF 797 kb]   (217 Downloads)    
Type of Study: Research | Subject: Special
Received: 2021/10/8 | Accepted: 2021/12/28 | Published: 2022/02/26

1. Hales CM, Carroll MD, Fryar CD, Ogden CL. Prevalence of obesity among adults and youth: United States, 2015-2016.
2. Ding S, Chi MM, Scull BP, Rigby R, Schwerbrock NM, Magness S, et al. High-fat diet: bacteria interactions promote intestinal inflammation which precedes and correlates with obesity and insulin resistance in mouse.Journal of PlOS ONE. 2010;5(8):e12191. [DOI:10.1371/journal.pone.0012191]
3. Kato S, Itoh K, Ochiai M, Iwai A, Park Y, Hata S, et al. Increased pentosidine, an advanced glycation end‐product, in urine and tissue reflects disease activity in inflammatory bowel diseases. Journal of gastroenterology and hepatology. 2008;23:S140-5. [DOI:10.1111/j.1440-1746.2008.05552.x]
4. Kim IW, Myung SJ, Do MY, Ryu YM, Kim MJ, Do EJ, et al. Western‐style diets induce macrophage infiltration and contribute to colitis‐associated carcinogenesis. Journal of gastroenterology and hepatology. 2010;25(11):1785-94. [DOI:10.1111/j.1440-1746.2010.06332.x]
5. Kellow NJ, Coughlan MT. Effect of diet-derived advanced glycation end products on inflammation. Nutrition Reviews. 2015;73(11):737-59. [DOI:10.1093/nutrit/nuv030]
6. Snelson M, Coughlan MT. Dietary advanced glycation end products: digestion, metabolism and modulation of gut microbial ecology. Nutrients. 2019;11(2):215. [DOI:10.3390/nu11020215]
7. van der Logt EM, Blokzijl T, van der Meer R, Faber KN, Dijkstra G. Westernized high-fat diet accelerates weight loss in dextran sulfate sodium-induced colitis in mice, which is further aggravated by supplementation of heme. The Journal of nutritional biochemistry. 2013;24(6):1159-65. [DOI:10.1016/j.jnutbio.2012.09.001]
8. Karlinger K, Györke T, Makö E, Mester Á, Tarján Z. The epidemiology and the pathogenesis of inflammatory bowel disease. European journal of radiology. 2000;35(3):154-67. [DOI:10.1016/S0720-048X(00)00238-2]
9. Anthoni C, Laukoetter MG, Rijcken E, Vowinkel T, Mennigen R, Muller S,et al. Mechanisms underlying the anti-inflammatory actions of boswellic acid derivatives in experimental colitis. American Journal of Physiology-Gastrointestinal and Liver Physiology. 2006;290(6):G1131-7. [DOI:10.1152/ajpgi.00562.2005]
10. Murakami A, Furukawa I, Miyamoto S, Tanaka T, Ohigashi H. Curcumin combined with turmerones, essential oil components of turmeric, abolishes inflammation‐associated mouse colon carcinogenesis. Biofactors. 2013;39(2):221-32. [DOI:10.1002/biof.1054]
11. Peterson CT, Vaughn AR, Sharma V, Chopra D, Mills PJ, Peterson SN, Sivamani RK. Effects of turmeric and curcumin dietary supplementation on human gut microbiota: A double-blind, randomized, placebo-controlled pilot study.Journal of Evidence-Based Integrative Medicine.2018;23. https://doi.org/10.1177/2515690X18790725 [DOI:10.1177/2515690X18790725.]
12. Yuan X, Sun H, Liu Y, Shiroshita T, Kawano S, Takeshi S, et al. Anti-cancer activity comparisons of aqueous extracts from Inonotus obliquus, Cordyceps militaris and Uncaria tomentosa in vitro and in vivo. Journal of Pharmacognosy and Phytochemistry. 2014;2(6).
13. Zhang L, Xue H, Zhao G, Qiao C, Sun X, Pang C, et al. Curcumin and resveratrol suppress dextran sulfate sodium induced colitis in mice. Molecular medicine reports. 2019;19(4):3053-60. [DOI:10.3892/mmr.2019.9974]
14. Alizadeh M, Kheirouri S. Curcumin against advanced glycation end products (AGEs) and AGEs-induced detrimental agents. Critical reviews in food science and nutrition. 2019;59(7):1169-77. [DOI:10.1080/10408398.2017.1396200]
15. Chilelli NC, Ragazzi E, Valentini R, Cosma C, Ferraresso S, Lapolla A, et al. Curcumin and boswellia serrata modulate the glyco-oxidative status and lipo-oxidation in master athletes. Nutrients. 2016;8(11):745. [DOI:10.3390/nu8110745]
16. Dil FA, Ranjkesh Z, Goodarzi MT. A systematic review of antiglycation medicinal plants. Diabetes & Metabolic Syndrome: Clinical Research & Reviews. 2019;13(2):1225-9. [DOI:10.1016/j.dsx.2019.01.053]
17. Starowicz M, Zieliński H. Inhibition of advanced glycation end-product formation by high antioxidant-leveled spices commonly used in European cuisine. Antioxidants. 2019;8(4):100. [DOI:10.3390/antiox8040100]
18. Vaughn AR, Branum A, Sivamani RK. Effects of turmeric (Curcuma longa) on skin health: a systematic review of the clinical evidence. Phytotherapy Research. 2016;30(8):1243-64. [DOI:10.1002/ptr.5640]
19. Zhu Y, Zhao Y, Wang P, Ahmedna M, Sang S. Bioactive ginger constituents alleviate protein glycation by trapping methylglyoxal. Chemical research in toxicology. 2015;28(9):1842-9. [DOI:10.1021/acs.chemrestox.5b00293]
20. Mahmoudi T, Abdolmohammadi K, Bashiri H, Mohammadi M, Rezaie MJ, Fathi F, et al. Hydrogen peroxide preconditioning promotes protective effects of umbilical cord vein mesenchymal stem cells in experimental pulmonary fibrosis. Advanced pharmaceutical bulletin. 2020;10(1):72. [DOI:10.15171/apb.2020.009]
21. Kim KA, Gu W, Lee IA, Joh EH, Kim DH. High fat diet-induced gut microbiota exacerbates inflammation and obesity in mice via the TLR4 signaling pathway.Journal of Plos one. 2012. https://doi.org/10.1371/journal.pone.0047713 [DOI:10.1371/journal.pone.0047713.]
22. Loftus Jr EV. Clinical epidemiology of inflammatory bowel disease: incidence, prevalence, and environmental influences. Gastroenterology. 2004;126(6):1504-17. [DOI:10.1053/j.gastro.2004.01.063]
23. Chuyen NV, Arai H, Nakanishi T, Utsunomiya N. Are food advanced glycation end products toxic in biological systems?. Annals of the New York Academy of Sciences. 2005;1043(1):467-73. [DOI:10.1196/annals.1333.053]
24. DeClercq V, McMurray DN, Chapkin RS. Obesity promotes colonic stem cell expansion during cancer initiation. Cancer letters. 2015;369(2):336-43. [DOI:10.1016/j.canlet.2015.10.001]
25. Vlassara H. Advanced glycosylation in nephropathy of diabetes and aging. Advances in nephrology from the Necker Hospital. 1996;25:303-15.
26. Harper JW, Zisman TL. Interaction of obesity and inflammatory bowel disease. World journal of gastroenterology. 2016;22(35):7868. [DOI:10.3748/wjg.v22.i35.7868]
27. Randhawa PK, Singh K, Singh N, Jaggi AS. A review on chemical-induced inflammatory bowel disease models in rodents. The Korean journal of physiology & pharmacology. 2014;18(4):279-88. [DOI:10.4196/kjpp.2014.18.4.279]
28. Luster AD. Chemokines-chemotactic cytokines that mediate inflammation. New England Journal of Medicine. 1998;338(7):436-45. [DOI:10.1056/NEJM199802123380706]

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