Volume 14, Issue 2 (volume 14, number 2 2022)                   IJDO 2022, 14(2): 105-109 | Back to browse issues page

XML Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Nikseresht F, Bahrami M, Rahmati M. The Effect of Interval Training on GCK Expression in Hepatocytes and Glucose Homeostasis in Type 2 Diabetes Rats. IJDO 2022; 14 (2) :105-109
URL: http://ijdo.ssu.ac.ir/article-1-707-en.html
Assistant Professor of Exercise Physiology, Sport Sciences Department, Faculty of Literature and Human Science, Lorestan University, Khorramabad, Iran.
Abstract:   (869 Views)
Objective: Hepatic glucose release plays a potential role in hyperglycemia in type 2 diabetes (T2D) patients. The aim of this experimental study was to determine the effect of 6 weeks of high-intensity interval training (HIIT) on fasting levels of glucose and insulin as well as glucokinase (GCK) expression in liver tissue in obese T2D rats.
Materials and Methods: T2D was induced by a high-fat diet (HFD) and streptozotocin (STZ) intraperitoneal injection in 14 male wistar rats, then were randomly divided into HIIT (n=7) and control (n=7) groups. The HIIT group practiced 6-week HIIT (5 days/ weekly). Finally, 48 hours after the last session, fasting levels of glucose, insulin, and GCK expression in liver hepatocytes of both groups were measured and compared by independent T-test (SPSS, Version 22.0).
Results: HIIT resulted in a significant decrease of fasting glucose compared to the control group (P< 0.0001). Compared with the control group, serum insulin (P: 0.018) and GCK expression in hepatocytes (P: 0.030) were significantly increased.
Conclusion: Based on these findings, the improvement in glucose in response to HIIT may be rooted in increased insulin and GCK expression in hepatocytes. However, understanding the mechanisms responsible for the effect of exercise training on the processes affecting hepatic glucose release requires further studies.
Full-Text [PDF 210 kb]   (180 Downloads)    
Type of Study: Research | Subject: Special
Received: 2022/02/18 | Accepted: 2022/04/8 | Published: 2022/05/20

1. Wang J, Chen C, Wang RY. Influence of short-and long-term treadmill exercises on levels of ghrelin, obestatin and NPY in plasma and brain extraction of obese rats. Endocrine. 2008;33(1):77-83. [DOI:10.1007/s12020-008-9056-z]
2. Mackelvie KJ, Meneilly GS, Elahi D, Wong AC, Barr SI, Chanoine JP. Regulation of appetite in lean and obese adolescents after exercise: role of acylated and desacyl ghrelin. The Journal of Clinical Endocrinology & Metabolism. 2007;92(2):648-54. [DOI:10.1210/jc.2006-1028]
3. Wang Z, Dong C. Gluconeogenesis in cancer: function and regulation of PEPCK, FBPase, and G6Pase. Trends in cancer. 2019;5(1):30-45. [DOI:10.1016/j.trecan.2018.11.003]
4. Zhang X, Yang S, Chen J, Su Z. Unraveling the regulation of hepatic gluconeogenesis. Frontiers in endocrinology. 2019:802. [DOI:10.3389/fendo.2018.00802]
5. Lin HV, Accili D. Hormonal regulation of hepatic glucose production in health and disease. Cell metabolism. 2011;14(1):9-19. [DOI:10.1016/j.cmet.2011.06.003]
6. De Souza CT, Frederico MJ, Da Luz G, Cintra DE, Ropelle ER, Pauli JR, et al. Acute exercise reduces hepatic glucose production through inhibition of the Foxo1/HNF‐4α pathway in insulin resistant mice. The Journal of physiology. 2010;588(12):2239-53. [DOI:10.1113/jphysiol.2009.183996]
7. Knudsen JG, Biensø RS, Hassing HA, Jakobsen AH, Pilegaard H. Exercise-induced regulation of key factors in substrate choice and gluconeogenesis in mouse liver. Molecular and cellular biochemistry. 2015;403(1):209-17. [DOI:10.1007/s11010-015-2351-0]
8. Gibala MJ. High intensity interval training: new insights. Sports Science Exchange. 2007;20(2):1-8.
9. Karimi M, Eizadi M. The effect of interval training on FOXO1 expression in pancreas tissue of diabetes rats with high fat diet and STZ. Razi Journal of Medical Sciences. 2019;26(6):95-104.(in Persian)
10. McAuley KA, Williams SM, Mann JI, Walker RJ, Lewis-Barned NJ, Temple LA, et al. Diagnosing insulin resistance in the general population. Diabetes care. 2001;24(3):460-4. [DOI:10.2337/diacare.24.3.460]
11. Cunha VN, de Paula Lima M, Motta‐Santos D, Pesquero JL, de Andrade RV, de Almeida JA, et al. Role of exercise intensity on GLUT4 content, aerobic fitness and fasting plasma glucose in type 2 diabetic mice. Cell biochemistry and function. 2015;33(7):435-42. [DOI:10.1002/cbf.3128]
12. Agius L. Glucokinase and molecular aspects of liver glycogen metabolism. Biochemical Journal. 2008;414(1):1-8. [DOI:10.1042/BJ20080595]
13. O'brien RM, Streeper RS, Ayala JE, Stadelmaier BT, Hornbuckle LA. Insulin-regulated gene expression. Biochemical Society Transactions. 2001;29(4):552-8. [DOI:10.1042/bst0290552]
14. Ramnanan CJ, Edgerton DS, Rivera N, Irimia-Dominguez J, Farmer B, Neal DW, et al. Molecular characterization of insulin-mediated suppression of hepatic glucose production in vivo. Diabetes. 2010;59(6):1302-11. [DOI:10.2337/db09-1625]

Add your comments about this article : Your username or Email:

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2023 CC BY-NC 4.0 | Iranian Journal of Diabetes and Obesity

Designed & Developed by : Yektaweb