Over Expression of FOXO1 in Subcutaneous Fatty Tissue and its Response to Resistance Training in High Fat Diet and Type 2 Diabetic Rat

Soheily, Shahram; Eizadi,  Mojtaba

doi:10.18502/ijdo.v14i2.9455

Volume 14, Issue 2 (5-2022) IJDO 2022, 14(2): 110-116 | Back to browse issues page

‎ 10.18502/ijdo.v14i2.9455

‎ 20.1001.1.20086792.2022.14.2.8.5

Mendeley

Zotero

RefWorks

Soheily S, Eizadi M. Over Expression of FOXO1 in Subcutaneous Fatty Tissue and its Response to Resistance Training in High Fat Diet and Type 2 Diabetic Rat. IJDO 2022; 14 (2) :110-116
URL: http://ijdo.ssu.ac.ir/article-1-708-en.html

Over Expression of FOXO1 in Subcutaneous Fatty Tissue and its Response to Resistance Training in High Fat Diet and Type 2 Diabetic Rat

Shahram Soheily ^*

, Mojtaba Eizadi

Assistant professor of Exercise Physiology, Safadasht Branch, Islamic Azad University, Tehran, Iran.

Abstract: (1018 Views)

Objective: Forkhead box proteins and Forkhead box transcription factor O1 (FOXO1) in particular, mediate insulin signaling pathways and glucose homeostasis. This study aimed to compare FOXO1 expression in subcutaneous adipose (SA) tissue between obese rats with and without type 2 diabetes (T2D) and its response to resistance training in T2D.
Materials and Methods: 21 male wistar rats (220±20 g) were obese by 6 weeks high fat diet (HFD) and randomly assigned to either non-diabetes (n=7) or two T2D groups (control and exercise groups, n=7 in each case). Fasting glucose, insulin, insulin resistance and FOXO1 expression were compared between non-diabetes and diabetes groups. All variables were also assigned after resistance training in the form of climbing a ladder (6 weeks/5 times weekly) in exercise compare with control groups. Data were compared by ANOVA, independent and paired t-test methods (P<0.05).
Results: Induction of diabetes resulted in significant increase in insulin resistance, glucose, and FOXO expression in SA tissue and a decrease in insulin compared to obese health rats (P< 0.0001). A significant decrease in fasting insulin (P< 0.0001), insulin resistance (P< 0.0001) and FOXO1 expression in SA tissue (P< 0.0001) and increase in insulin (P: 0.002) were observed by resistance training compared to control diabetes rats.
Conclusion: Based on our results, improving insulin resistance and glucose in response to resistance training in obese diabetic rats may be rooted in decreased insulin expression following these exercises.

Keywords: Adipose tissue, Exercise, Insulin resistance, Diabetes

Full-Text [PDF 675 kb] (412 Downloads)

Type of Study: Research | Subject: Special
Received: 2021/11/25 | Accepted: 2022/03/20 | Published: 2022/05/20

References

1. Dowell P, Otto TC, Adi S, Lane MD. Convergence of peroxisome proliferator-activated receptor γ and Foxo1 signaling pathways. Journal of Biological Chemistry. 2003;278(46):45485-91. [DOI:10.1074/jbc.M309069200]

2. Tontonoz P, Spiegelman BM. Fat and beyond: The diverse biology of PPARgamma. Annual Review of Biochemistry. 2008; 77: 289-312. [DOI:10.1146/annurev.biochem.77.061307.091829]

3. Klöting N, Schleinitz D, Ruschke K, Berndt J, Fasshauer M, Tönjes A, et al. Inverse relationship between obesity and FTO gene expression in visceral adipose tissue in humans. Diabetologia. 2008;51(4):641-7. [DOI:10.1007/s00125-008-0928-9]

4. Cha SW, Choi SM, Kim KS, Park BL, Kim JR, Kim JY, et al. Replication of genetic effects of FTO polymorphisms on BMI in a Korean population. Obesity. 2008;16(9):2187-9. [DOI:10.1038/oby.2008.314]

5. Wang Y, Zhou Y, Graves DT. FOXO transcription factors: their clinical significance and regulation. BioMed research international, 2014; 2014: 925350. [DOI:10.1155/2014/925350]

6. Nagashima T, Shigematsu N, Maruki R, Urano Y, Tanaka H, Shimaya A, et al. Discovery of novel forkhead box O1 inhibitors for treating type 2 diabetes: improvement of fasting glycemia in diabetic db/db mice. Molecular pharmacology. 2010;78(5):961-70. [DOI:10.1124/mol.110.065714]

7. Sanchez AM. FoxO transcription factors and endurance training: a role for FoxO1 and FoxO3 in exercise-induced angiogenesis. The Journal of physiology. 2015;593(Pt 2):363. [DOI:10.1113/jphysiol.2014.285999]

8. Azad M, Khaledi N, Hedayati M. Effect of acute and chronic eccentric exercise on FOXO1 mRNA expression as fiber type transition factor in rat skeletal muscles. Gene. 2016 ;584(2):180-4. [DOI:10.1016/j.gene.2016.02.033]

9. Karimi M, Eizadi M. The effect of interval training on FOXO1 expression in pancreas tissue of diabetes rats with high fat diet and STZ. Razi Journal of Medical Sciences. 2019 10;26(6):95-104.(in Persian)

10. Yazdanpazhooh S, Banaeifar A, Arshadi S, Eizadi M. Six weeks resistance training effect on FTO expression in type II diabetes rats. Iranian Journal of Diabetes and Obesity. 2018;10(4):216-22.

11. Eizadi M, Ravasi AA, Soory R, Baesi K, Choobineh S. The effect of three months of resistance training on TCF7L2 expression in pancreas tissues of type 2 diabetic rats. Avicenna Journal of Medical Biochemistry. 2016;4(1):12-34014. [DOI:10.17795/ajmb-34014]

12. Eizadi M, Soory R, Ravasi A, Baesy K, Choobineh S. Relationship between TCF7L2 relative expression in pancreas tissue with changes in insulin by high intensity interval training (HIIT) in type 2 diabetes rats. Journal of Shahid Sadoughi University of Medical Sciences. 2017;24 (12): 981-993. (in Persian)

13. Marita AR, Sarkar JA, Rane S. Type 2 diabetes in non-obese Indian subjects is associated with reduced leptin levels: study from Mumbai, Western India. Molecular and Cellular Biochemistry. 2005;275(1):143-51. [DOI:10.1007/s11010-005-1204-7]

14. Coughlin CC, Finck BN, Eagon JC, Halpin VJ, Magkos F, Mohammed BS, et al. Effect of marked weight loss on adiponectin gene expression and plasma concentrations. Obesity. 2007;15(3):640-5. [DOI:10.1038/oby.2007.556]

15. Ruchat SM, Rankinen T, Weisnagel SJ, Rice T, Rao DC, Bergman RN, et al. Improvements in glucose homeostasis in response to regular exercise are influenced by the PPARG Pro12Ala variant: results from the Heritage Family Study. Diabetologia. 2010;53(4):679-89. [DOI:10.1007/s00125-009-1630-2]

16. Kawano Y, Nakae J, Watanabe N, Fujisaka S, Iskandar K, Sekioka R, et al. Loss of Pdk1-Foxo1 signaling in myeloid cells predisposes to adipose tissue inflammation and insulin resistance. Diabetes. 2012 ;61(8):1935-48. [DOI:10.2337/db11-0770]

17. Schick EE. The effect of FoxO1 on glycemic control and skeletal muscle glucose uptake and lipid metabolism. The University of Toledo; 2014:1642. [DOI:10.1249/01.mss.0000466148.15483.b5]

18. Fan W, Imamura T, Sonoda N, Sears DD, Patsouris D, Kim JJ, et al. FOXO1 transrepresses peroxisome proliferator-activated receptor γ transactivation, coordinating an insulin-induced feed-forward response in adipocytes. Journal of biological chemistry. 2009;284(18):12188-97. [DOI:10.1074/jbc.M808915200]

19. Xu Y, Jin B, Sun L, Yang H, Cao X, Zhang G. The expression of FoxO1 in placenta and omental adipose tissue of gestational diabetes mellitus. Experimental and Clinical Endocrinology & Diabetes. 2014;122(05):287-94. [DOI:10.1055/s-0034-1371830]

20. Nakae J, Cao Y, Hakuno F, Takemori H, Kawano Y, Sekioka R, et al. Novel repressor regulates insulin sensitivity through interaction with Foxo1. The European Molecular Biology Organization Journal. 2012; 31(10):2275-95. [DOI:10.1038/emboj.2012.97]

21. Slopack D, Roudier E, Liu ST, Nwadozi E, Birot O, Haas TL. Forkhead BoxO transcription factors restrain exercise‐induced angiogenesis. The Journal of physiology. 2014 ;592(18):4069-82. [DOI:10.1113/jphysiol.2014.275867]

22. Yarmohammadi M, Behboudi L, Eizadi M. The Effect of 12 Weeks Resistance Training on FOXO1 Expression in Hepatocytes, Glucose and Insulin in Diabetic Rats-A Brief-Report. Iranian journal of diabetes and obesity. 2019;11(3):193-5. [DOI:10.18502/ijdo.v11i3.2611]

23. Izadi M. Investigation effect of 12-week high-intensity interval training FOXO1 gene expression of subcutaneous adipose tissue and insulin resistance in type 2 diabetic rats. Journal of Neyshabur University of Medical Sciences. 2018; 6(19): 12-20.(in Persian)