The Effect of Combined Exercises and Consumption of Mulberry Leaf Extract on Serum Inflammatory Markers Level in Elderly Type 2 Diabetes Mellitus Men

Bahram, Mohammad Ebrahim; Afroundeh, Roghayyeh; Pourvaghar, Mohammad Javad; Seify Skishahr, Farnaz; Katebi, Leila; Isik, Ozkan

doi:10.18502/ijdo.v15i3.13733

Volume 15, Issue 3 (volume 15, number 3 2023) IJDO 2023, 15(3): 129-138 | Back to browse issues page

‎ 10.18502/ijdo.v15i3.13733

Mendeley

Zotero

RefWorks

Bahram M E, Afroundeh R, Pourvaghar M J, Seify Skishahr F, Katebi L, Isik O. The Effect of Combined Exercises and Consumption of Mulberry Leaf Extract on Serum Inflammatory Markers Level in Elderly Type 2 Diabetes Mellitus Men. IJDO 2023; 15 (3) :129-138
URL: http://ijdo.ssu.ac.ir/article-1-813-en.html

The Effect of Combined Exercises and Consumption of Mulberry Leaf Extract on Serum Inflammatory Markers Level in Elderly Type 2 Diabetes Mellitus Men

Mohammad Ebrahim Bahram

, Roghayyeh Afroundeh ^*

, Mohammad Javad Pourvaghar

, Farnaz Seify Skishahr

, Leila Katebi

, Ozkan Isik

Associate Professor of Sports Physiology, Faculty of Educational Sciences and Psychology, University of Mohaghegh Ardabili, Ardabil, Iran.

Abstract: (188 Views)

Objective: This study aimed to investigate the effect of 8 weeks combined exercise and consumption of Mulberry leaf extract on the serum levels of inflammatory and atherosclerosis indicators in elderly men with type 2 diabetes mellitus (T2DM).
Materials and Methods: 40 elderly men T2DM aged between 65 to 70 years old were purposefully selected and randomly divided into five equal groups (training, supplement, training+ supplement, placebo, and control). A daily dose of 1000 mg Mulberry leaf extract, 3 times a day, was used for 2 months by the supplement group. Training groups performed combined exercises for eight weeks, each week three sessions of 90 minutes.
Results: The results showed that the amount of salusin-β and interleukin-6 in the training, training + supplement and supplement groups decreased significantly at the end of the study; Meanwhile, the level of salusin-α increased significantly in the training (P= 0.001), training + supplement (P= 0.001) and supplement (P= 0.01) groups. Also, the results of the covariance analysis showed that the amount of salusin-β and interleukin 6 were lower (P= 0.001), and the level of salusin-α was higher significantly in the training (P= 0.001), training +supplement (P= 0.001), and supplement (P= 0.001) groups compared to the control groups.
Conclusion: It seems that the consumption of Mulberry leaf extract, and combined exercises, is effective in controlling the inflammatory indicators and atherosclerosis related to diabetes in the T2DM elderly.

Corresponding Author: Roghayyeh Afroundeh
View Orcid in Profile
You can search for this author in PubMed Google Scholar Profile

Keywords: Interleukin 6, Salusin-α, Salusin-β, Diabetes, Elderly

Full-Text [PDF 276 kb] (102 Downloads)

Type of Study: Research | Subject: Special
Received: 2023/04/29 | Accepted: 2023/09/5 | Published: 2023/09/30

References

1. Thomas GN, Jiang CQ, Taheri S, Xiao ZH, Tomlinson B, Cheung BM, et al. A systematic review of lifestyle modification and glucose intolerance in the prevention of type 2 diabetes. Curr Diabetes Rev. 2010;6(6):378-87.

2. Khalangot M, Tronko M, Kravchenko V, Kulchinska J, Hu G. Body mass index and the risk of total and cardiovascular mortality among patients with type 2 diabetes: a large prospective study in Ukraine. Heart. 2009;95(6):454-60.

3. Watanabe T, Nishio K, Kanome T, Matsuyama TA, Koba S, Sakai T, et al. Impact of salusin-α and-β on human macrophage foam cell formation and coronary atherosclerosis. Circulation. 2008;117(5):638-48.

4. Zhao MX, Zhou B, Ling L, Xiong XQ, Zhang F, Chen Q, et al. Salusin-β contributes to oxidative stress and inflammation in diabetic cardiomyopathy. Cell death & disease. 2017;8(3):e2690.

5. Guilherme A, Virbasius JV, Puri V, Czech MP. Adipocyte dysfunctions linking obesity to insulin resistance and type 2 diabetes. Nature reviews Molecular cell biology. 2008;9(5):367-77.

6. Yaturu S, Rains J, Jain SK. Relationship of elevated osteoprotegerin with insulin resistance, CRP, and TNF-α levels in men with type 2 diabetes. Cytokine. 2008;44(1):168-71.

7. Fujie S, Hasegawa N, Sanada K, Hamaoka T, Maeda S, Padilla J, et al. Increased serum salusin-α by aerobic exercise training correlates with improvements in arterial stiffness in middle-aged and older adults. Aging (Albany NY). 2020;12(2):1201.

8. Kimura T, Nakagawa K, Kubota H, Kojima Y, Goto Y, Yamagishi K, et al. Food-grade mulberry powder enriched with 1-deoxynojirimycin suppresses the elevation of postprandial blood glucose in humans. Journal of agricultural and food chemistry. 2007;55(14):5869-74.

9. Kim JY, Chung HI, Jung KO, Wee JH, Kwon O. Chemical profiles and hypoglycemic activities of mulberry leaf extracts vary with ethanol concentration. Food Science and Biotechnology. 2013;22:1-5. [DOI:10.1007/s10068-013-0233-3]

10. Paahoo A, Tadibi V, Behpoor N. Effect of two chronic exercise protocols on pre-atherosclerotic and anti-atherosclerotic biomarkers levels in obese and overweight children. Iranian journal of pediatrics. 2020;30(2):e99760.

11. Nazari M, Minasian V, Hovsepian S. Effects of two types of moderate-and high-intensity interval training on serum salusin-α and salusin-β levels and lipid profile in women with overweight/obesity. Diabetes, Metabolic Syndrome and Obesity. 2020;13:1385-90.

12. Bahram ME, Afroundeh R, Pourvaghar M, Seify F, Katebi L. The Effect of Mulberry leaf Extract Supplementation and Combined Exercises on Lipocalin-2, Interleukin 1 beta Levels and Diabetes-Related Metabolic Parameters in Elderly Patients With Type 2 Diabetes. Journal of Sport Biosciences. 2022;14(3): 31-49.(in Persian)

13. Touvra AM, Volaklis KA, Spassis AT, Zois CE, Douda HT, Kotsa K, et al. Combined strength and aerobic training increases transforming growth factor-β1 in patients with type 2 diabetes. Hormones. 2011;10:125-30.

14. Shenoy S, Guglani R, Sandhu JS. Effectiveness of an aerobic walking program using heart rate monitor and pedometer on the parameters of diabetes control in Asian Indians with type 2 diabetes. Primary Care Diabetes. 2010;4(1):41-5.

15. Ghiyami Sh, Afroundeh R, Pourvaghar Mj, Sadeghi A, Katebi L. The Effect Of 8 Weeks Combined Training Along With The Use Of Mulberry Leaf Extract On Serum Levels Of Fetuin A And Fetuin B Levels In Elderly Men With Type Diabetes 2. Studies In Medical Sciences. 2022;33(6):426-40.(inPersian)

16. Colberg SR, Sigal RJ, Yardley JE, Riddell MC, Dunstan DW, Dempsey PC, et al. Physical activity/exercise and diabetes: a position statement of the American Diabetes Association. Diabetes care. 2016;39(11):2065-79.

17. Ferriolli E, Pessanha FP, Marchesi JC. Diabetes and exercise in the elderly. Diabetes and physical activity. Medicine and Sport Science . 2014;60:122-9.

18. Riche DM, Riche KD, East HE, Barrett EK, May WL. Impact of mulberry leaf extract on type 2 diabetes (Mul-DM): a randomized, placebo-controlled pilot study. Complementary Therapies in Medicine. 2017;32:105-8.

19. Aliniya N, Elmieh A, Fadaei Chafy MR. Interaction effect of combined exercise and supplementation with portulaca oleracea on liver enzymes in obese postmenopausal women with non-alcoholic fatty liver disease. Complementary Medicine Journal. 2020;10(1):68-79.(in Persian)

20. Ghaffarpour M, Houshiar-Rad A, Kianfar HJ. The manual for household measures, cooking yields factors and edible portion of foods. Tehran: Nashre Olume Keshavarzy. 1999;7(213):42-58.

21. Jackson AS, Pollock ML. Generalized equations for predicting body density of men. British journal of nutrition. 1978;40(3):497-504.

22. Zhu X, Zhou Y, Cai W, Sun H, Qiu L. Salusin-β mediates high glucose-induced endothelial injury via disruption of AMPK signaling pathway. Biochemical and biophysical research communications. 2017;491(2):515-21.

23. Nikseresht H, Tadibi V, Behpoor N. The Effects of 8 Weeks of Aerobic and Resistance Exercises on Salusins Levels and Inflammatory Indices in Type 2 Diabetic Women. Sport Physiology. 2020;12(47):73-92.(in Persian)

24. Xu X, He M, Liu T, Zeng Y, Zhang W. Effect of salusin-ß on peroxisome proliferator-activated receptor gamma gene expression in vascular smooth muscle cells and its possible mechanism. Cellular Physiology and Biochemistry. 2015;36(6):2466-79.

25. Verrier E, Wang L, Wadham C, Albanese N, Hahn C, Gamble JR, et al. PPARγ agonists ameliorate endothelial cell activation via inhibition of diacylglycerol-protein kinase C signaling pathway: role of diacylglycerol kinase. Circulation research. 2004;94(11):1515-22.

26. Nakayama C, Shichiri M, Sato K, Hirata Y. Expression of prosalusin in human neuroblastoma cells. Peptides. 2009;30(7):1362-7.

27. Guilluy C, Brégeon J, Toumaniantz G, Rolli-Derkinderen M, Retailleau K, Loufrani L, et al. The Rho exchange factor Arhgef1 mediates the effects of angiotensin II on vascular tone and blood pressure. Nature medicine. 2010;16(2):183-90.

28. Sun HJ, Chen D, Wang PY, Wan MY, Zhang CX, Zhang ZX, et al. Salusin-β is involved in diabetes mellitus-induced endothelial dysfunction via degradation of peroxisome proliferator-activated receptor gamma. Oxidative medicine and cellular longevity. 2017;2017.

29. Koya T, Miyazaki T, Watanabe T, Shichiri M, Atsumi T, Kim-Kaneyama JR, et al. Salusin-β accelerates inflammatory responses in vascular endothelial cells via NF-κB signaling in LDL receptor-deficient mice in vivo and HUVECs in vitro. American Journal of Physiology-Heart and Circulatory Physiology. 2012;303(1):H96-105.

30. Atashak S, Niloofari A, Azizbaygi K. The effect of blackberry extract on the total antioxidant capacity and lipid peroxidation after acute resistance exercise in obese men. Journal of Food Technology and Nutrition. 2014;11(2):55-62.(in Persian)

31. Grzegorzewska AE, Niepolski L, Sikora J, Janków M, Jagodzinski PP, Sowinska A. Effect of lifestyle changes and atorvastatin administration on dyslipidemia in hemodialysis patients: a prospective study. Pol Arch Med Wewn. 2014;124(9):443-51.

32. Tofas T, Fatouros IG, Draganidis D, Deli CK, Chatzinikolaou A, Tziortzis C, et al. Effects of cardiovascular, resistance and combined exercise training on cardiovascular, performance and blood redox parameters in coronary artery disease patients: An 8-month training-detraining randomized intervention. Antioxidants. 2021;10(3):409.

33. Khalighfard S, Gaeini A, Nazarali P. The effect of endurance exercise on cardiac stress and exercise induced immune response in elite kayakers. Razi Journal of Medical Sciences. 2011;17(80):8-15.(in Persian)

34. Steptoe A, Hamer M, Chida Y. The effects of acute psychological stress on circulating inflammatory factors in humans: a review and meta-analysis. Brain, behavior, and immunity. 2007;21(7):901-12.

35. Radom-Aizik S, Leu SY, Cooper DM, Zaldivar Jr F. Serum from exercising humans suppresses t-cell cytokine production. Cytokine. 2007;40(2):75-81.

36. Takikawa M, Inoue S, Horio F, Tsuda T. Dietary anthocyanin-rich bilberry extract ameliorates hyperglycemia and insulin sensitivity via activation of AMP-activated protein kinase in diabetic mice. The Journal of nutrition. 2010;140(3):527-33.

37. Zhishen J, Mengcheng T, Jianming W. The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food chemistry. 1999;64(4):555-9.

38. Bahram Me, Afroundeh R, Pourvaghar Mj, Seyfi F, Katebi L. Studying The Simultaneous Effect Of Combined Exercises And Supplementation Of Mulberry Leaf Extract On Some Inflammatory Biomarkers Effective In The Pathogenesis Of Diabetes And Hba1c In Elderly Men With Type 2 Diabetes. Studies in Medical Sciences. 2022;33(5):322-35.(in Persian)

39. Kessler HS, Sisson SB, Short KR. The potential for high-intensity interval training to reduce cardiometabolic disease risk. Sports medicine. 2012;42:489-509.

40. Poehlman ET, Dvorak RV, DeNino WF, Brochu M, Ades PA. Effects of resistance training and endurance training on insulin sensitivity in nonobese, young women: a controlled randomized trial. The Journal of Clinical Endocrinology & Metabolism. 2000;85(7):2463-8. [DOI:10.1210/jcem.85.7.6692]

41. Tolouei Azar J, Shabkhiz F, Khalafi M. The effects of eight weeks of resistance training on serum levels IL-15, IL-6, TNF-α and insulin resistance in older type 2 diabetic men. Journal of Sport Biosciences. 2021;12(4):391-406.(in Persian)

42. Ghafari M, Banitalebi E, Heidari A. Impact of High-Intensity Interval Training and at Concurrent Strength-Endurance Training on the Levels of Some Adipokines Associated with Insulin Resistance in Women with Diabetes Mellitus. Health Research Journal. 2017;2(3):193-206.