Iranian Journal of Diabetes and Obesity
Shahid Sadoughi University Of Medical Sciences
Sun, Dec 22, 2024
[Archive]
Volume 15, Issue 3 (10-2023)
IJDO 2023, 15(3): 190-193 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Ghofrani M H, Rahimi A, Alijani E, Feizolahi F. The Impact of Resistance and Aerobic Training on the Insulin Synthesis Genes Expression in Diabetic Rats. IJDO 2023; 15 (3) :190-193
URL: http://ijdo.ssu.ac.ir/article-1-820-en.html
URL: http://ijdo.ssu.ac.ir/article-1-820-en.html
Associate professor of Exercise Physiology, Department of Physical Education and Sport Sciences, Karaj Branch, Islamic Azad University, Karaj, Iran.
Abstract: (377 Views)
Objective: In the last two decades, genetic studies have strongly supported the effective role of genetic factors on the synthesis of insulin from the pancreas. The purpose of this research is to determine the effect of aerobic and resistance exercises on the expression of NeruoD1 and PDX1 in pancreatic tissue, as well as serum insulin and glucose levels in type 2 diabetic rats.
Materials and Methods: 21 male wistar rats (220±10 g) were included. Then diabetic rats were randomly assigned into 3 groups: 1) control (no-training), 2) resistance training and 3) aerobic training. Exercise training lasted 10 weeks and 5 times weekly for training groups. After intervention, NeruoD1 and PDX1 expression in pancreas, insulin and glucose were compared between groups. Data compared by one way ANOVA and Tukey's post hoc test between groups (P < 0.05).
Results: Compared to control rats, resistance and aerobic training led to significant increase in serum insulin (P: 0.001, P: 0.013 respectively), PDX1 expression (P: 0.001, P: 0.001 respectively) and decrease glucose (P: 0.001, P: 0.001 respectively). Significant difference was not observed between control and aerobic groups with regard to NeruoD1 expression (P: 0.077). In addition, NeruoD1 expression in resistance group was significantly higher than aerobic and control groups (P: 0.018, P: 0.001, respectively).
Conclusion: Despite the improvement of insulin and glucose in response to both aerobic and resistance training, it seems that resistance training affects genes affecting insulin synthesis and transcription more than aerobic training.
Materials and Methods: 21 male wistar rats (220±10 g) were included. Then diabetic rats were randomly assigned into 3 groups: 1) control (no-training), 2) resistance training and 3) aerobic training. Exercise training lasted 10 weeks and 5 times weekly for training groups. After intervention, NeruoD1 and PDX1 expression in pancreas, insulin and glucose were compared between groups. Data compared by one way ANOVA and Tukey's post hoc test between groups (P < 0.05).
Results: Compared to control rats, resistance and aerobic training led to significant increase in serum insulin (P: 0.001, P: 0.013 respectively), PDX1 expression (P: 0.001, P: 0.001 respectively) and decrease glucose (P: 0.001, P: 0.001 respectively). Significant difference was not observed between control and aerobic groups with regard to NeruoD1 expression (P: 0.077). In addition, NeruoD1 expression in resistance group was significantly higher than aerobic and control groups (P: 0.018, P: 0.001, respectively).
Conclusion: Despite the improvement of insulin and glucose in response to both aerobic and resistance training, it seems that resistance training affects genes affecting insulin synthesis and transcription more than aerobic training.
Type of Study: Research |
Subject:
Special
Received: 2023/05/1 | Accepted: 2023/08/5 | Published: 2023/09/30
Received: 2023/05/1 | Accepted: 2023/08/5 | Published: 2023/09/30
References
1. Adeghate E, Schattner P, Dunn E. An update on the etiology and epidemiology of diabetes mellitus. Annals of the New York academy of sciences. 2006;1084(1):1-29.
2. Ruchat SM, Rankinen T, Weisnagel SJ, Rice T, Rao DC, Bergman RN, Bouchard C, Perusse L. Improvements in glucose homeostasis in response to regular exercise are influenced by the PPARG Pro12Ala variant: results from the Heritage Family Study. Diabetologia. 2010;53(4):679-89.
3. Fu Z, R Gilbert E, Liu D. Regulation of insulin synthesis and secretion and pancreatic Beta-cell dysfunction in diabetes. Current diabetes reviews. 2013;9(1):25-53.
5. Grant SF, Thorleifsson G, Reynisdottir I, Benediktsson R, Manolescu A, Sainz J, et al. Variant of transcription factor 7-like 2 (TCF7L2) gene confers risk of type 2 diabetes. Nature genetics. 2006;38(3):320-3.
6. Kaneto H, Matsuoka TA. Role of pancreatic transcription factors in maintenance of mature β-cell function. International journal of molecular sciences. 2015;16(3):6281-97.
7. Matsuoka TA, Kaneto H, Kawashima S, Miyatsuka T, Tochino Y, Yoshikawa A, et al. Preserving Mafa expression in diabetic islet β-cells improves glycemic control in vivo. Journal of Biological Chemistry. 2015;290(12):7647-57.
8. Kaneto H. Pancreatic β-cell glucose toxicity in type 2 diabetes mellitus. Current Diabetes Reviews. 2015;11(1):2-6.
9. Eizadi M, Ravasi AA, Soory R, Baesi K, Choobineh S. The effect of three months of resistance training on TCF7L2 expression in pancreas tissues of type 2 diabetic rats. Avicenna Journal of Medical Biochemistry. 2016;4(1):12-34014.
10. Ramazani Rad M, Hajirasouli M, Eizadi M. The effect of 12 weeks of aerobic training on GLP-1 receptor expression in pancreatic tissue and glycemic control in type 2 diabetic rats. Qom University of Medical Sciences Journal. 2017;11(6):36-45.(in Persian)
11. Zhang C, Moriguchi T, Kajihara M, Esaki R, Harada A, Shimohata H, et al. MafA is a key regulator of glucose-stimulated insulin secretion. Molecular and cellular biology. 2005;25(12):4969-76.
12. Brelje TC, Stout LE, Bhagroo NV, Sorenson RL. Distinctive roles for prolactin and growth hormone in the activation of signal transducer and activator of transcription 5 in pancreatic islets of langerhans. Endocrinology. 2004;145(9):4162-75.
13. Nishimura W, Yao I, Iida J, Tanaka N, Hata Y. Interaction of synaptic scaffolding molecule and β-catenin. Journal of Neuroscience. 2002;22(3):757-65.
14. Eizadi M, Soory R, Ravasi A, Baesy K, Choobineh S. Relationship between TCF7L2 relative expression in pancreas tissue with changes in insulin by high intensity interval training (HIIT) in type 2 diabetes rats.Journal of Shahid Sadoughi University of Medical Sciences .2017;24(12):981-93.(in Persian)
15. Ghahramani M, Banaei Far, Arshadi, Sohaily. Effect Of Aerobic Training On Expression Of Pgc-1α & Pepck Genes In Hepatocyte Of Streptozotocin-Induced Diabetic Male Rats. Studies In Medical Sciences 2019; 30 (10) :791-802.(in Persian)
16. Simões e Silva LL, Santos de Sousa Fernandes M, Kubrusly MS, Muller CR, Américo AL, Stefano JT, et al. Effects of aerobic exercise protocol on genes related to insulin resistance an inflammation in the pancreas of Ob/Ob mice with NAFLD. Clinical and Experimental Gastroenterology. 2020;13:223-34.
17. Yazdanpazhooh S, Banaeifar A, Arshadi S, Eizadi M. Six weeks resistance training effect on FTO expression in type II diabetes rats. Iranian Journal of Diabetes and Obesity. 2018;10(4):216-2.
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
