Effect of High-Intensity Interval Training on TCF7L2 Gene Expression in Hepatocytes of Obese Rats

Karimy, Akbar; Kazemzadeh, Yaser; Eizadi, Mojtaba; Sedaghaty, Saeid; Ghotnian, Sepideh

doi:10.18502/ijdo.v16i1.15237

Volume 16, Issue 1 (volume 16, number 1 2024) IJDO 2024, 16(1): 17-24 | Back to browse issues page

‎ 10.18502/ijdo.v16i1.15237

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Karimy A, Kazemzadeh Y, Eizadi M, Sedaghaty S, Ghotnian S. Effect of High-Intensity Interval Training on TCF7L2 Gene Expression in Hepatocytes of Obese Rats. IJDO 2024; 16 (1) :17-24
URL: http://ijdo.ssu.ac.ir/article-1-851-en.html

Effect of High-Intensity Interval Training on TCF7L2 Gene Expression in Hepatocytes of Obese Rats

Akbar Karimy

, Yaser Kazemzadeh ^*

, Mojtaba Eizadi

, Saeid Sedaghaty

, Sepideh Ghotnian

Assistant Professor of Exercise Physiology, Islamshahr Branch, Islamic Azad University, Tehran, Iran.

Abstract: (79 Views)

Objective Hepatic glucose release is greatly increased in the presence of obesity and related diseases. The research objective was to explore the impact of high intensity interval training (HIIT) on TCF7L2 gene expression in hepatocytes of obese rats.
Materials and Methods: Out of 21 male Wistar rats aged 10 weeks years (220±10 g), obesity was induced in 14 rats by 8-week high-fat diet. The rats were then divided into normal (n=7), obese control (n=7), and HIIT obese (n=7) groups. Rats in the HIIT group completed 8 weeks of HIIT/5 days weekly, whereas the other groups were inactive. After intervention, TCF7L2 gene expression in hepatocytes, insulin resistance and glucose compared using ANOVA /Tukey’s post hoc test between groups by SPSS-22.
Results: Obesity induction led to a significant decrease in TCF7L2 gene expression (P: 0.011) and an increase in blood glucose (P: 0.009) and insulin resistance (P: 0.019) compared with the normal group (P< 0.001). On the other hand, interval training led to a significant increase in TCF7L2 gene expression (P: 0.029) and a decrease in blood glucose (P< 0.001) and insulin resistance (P< 0.001) in the obese group compared with the control group.
Conclusion: The observed enhancement in fasting blood glucose levels among obese rats could be linked to increased TCF7L2 gene expression in liver cells, which appears to be a response to interval training intervention. Nevertheless, understanding the main mechanisms responsible for observed changes requires further studies in this field.

Keywords: TCF7L2 expression, Hepatocytes, Interval training, Obesity, Gluconeogenesis

Full-Text [PDF 584 kb] (40 Downloads)

Type of Study: Research | Subject: Special
Received: 2023/12/6 | Accepted: 2024/02/3 | Published: 2024/03/20

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