Effect of Different Training Methods on SIRT1 Gene Expression in Subcutaneous Adipose Tissue of Male Wistar Rats

Tavasoli, Amirhossein; Shariatzadeh Joneydi, Mohammad; Ramezani, Javad

doi:10.18502/ijdo.v17i4.20039

Volume 17, Issue 4 (10-2025) IJDO 2025, 17(4): 248-255 | Back to browse issues page

‎ 10.18502/ijdo.v17i4.20039

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Tavasoli A, Shariatzadeh Joneydi M, Ramezani J. Effect of Different Training Methods on SIRT1 Gene Expression in Subcutaneous Adipose Tissue of Male Wistar Rats. IJDO 2025; 17 (4) :248-255
URL: http://ijdo.ssu.ac.ir/article-1-992-en.html

Effect of Different Training Methods on SIRT1 Gene Expression in Subcutaneous Adipose Tissue of Male Wistar Rats

Amirhossein Tavasoli

, Mohammad Shariatzadeh Joneydi

, Javad Ramezani ^*

Assistant Professor, Department of Sport Sciences, Payam Noor University, Tehran, Iran.

Abstract: (121 Views)

Objective: SIRT1 is a key regulator of adipose tissue's energy balance, inflammation, and mitochondrial function. This study was designed to investigate the effect of different training modalities, Moderate-Intensity Aerobic Training (MIAT), High-Intensity Aerobic Training (HIAT), and High-Intensity Interval Training (HIIT), on SIRT1 gene expression in subcutaneous adipose tissue of male Wistar rats.
Materials and Methods: Thirty-two 8-week-old male Wistar rats (237 ± 33 g) were randomly assigned to four groups (n= 8): Control, MIAT, HIAT, and HIIT. Animals were housed under controlled conditions and had free access to food and water. Training protocols were performed five times per week for eight weeks. After 12 hours of fasting and 24 hours post-intervention, subcutaneous abdominal adipose tissue was collected. SIRT1 gene expression was analyzed using RT-PCR. Data were statistically evaluated using one-way ANOVA and LSD post hoc test (P< 0.05) in SPSS-22.
Results: The results showed that all three training models - MIAT (P= 0.023), HIAT (P= 0.001), and HIIT (P= 0.003) - significantly increased SIRT1 gene expression compared to the control group.
Conclusion: Although all training modalities significantly increased SIRT1 gene expression compared to the control group, the HIAT group showed the highest mean expression level. However, no statistically significant difference was observed between the training groups.

Keywords: Aerobic exercise training, Adipose tissue, Gene expression, SIRT1

Full-Text [PDF 447 kb] (65 Downloads)

Type of Study: Research | Subject: Special
Received: 2025/04/25 | Accepted: 2025/10/21 | Published: 2025/10/30

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